过敏性鼻炎-哮喘综合征大鼠模型的建立与评价

Establishment of Aats Allergic Rhinitis and Asthma Syndrome Model and Evaluation

目的:建立一种大鼠过敏性鼻炎-哮喘综合征的模型并评价其效果。方法:清洁级健康雄性SD大鼠20只,随机分为对照组和模型组,运用卵白蛋白腹腔注射14天后,用卵白蛋白连续鼻部滴注7天,从第15日开始,将模型组大鼠置于密闭器皿内(自制的50cm×30cm×20cm透明带盖塑料盒),给予2%OVA溶液进行雾化吸入,建立模型;对照组以生理盐水代替。结果:模型组经抗原激发后张口喘息,腹部翕动,易激惹,躁动不安,抖毛,抓鼻,打喷嚏,甚则出现口唇及双耳紫绀,饮水量明显增多,精神萎靡,食量减少,反应迟钝,活动明显减少或俯卧不动,毛色发黄卷曲,枯槁无华等哮喘急性发作表现。BALF(支气管肺泡灌洗液)中白细胞总数及嗜酸性粒细胞分类明显增多。鼻部及肺组织病理切片见小支气管及伴行血管周围有较多炎症细胞,可见大量嗜酸性粒细胞细胞浸润。结论:经探索建立的过敏性鼻炎-哮喘综合征模型其发病机理与临床相似,这种成功的制模方法有一定的推广价值。

Objective：To establish a rat model of allergic rhinitis and asthma syndrome and evaluate its effect.Methods：Clean level 20 healthy male SD rats,were randomly divided into control group and model group,14 days after the use of intraperitoneal injection of ovalbumin,continuous nasal drip with ovalbumin 7 days,starting from 15th,the model group rats in the airtight vessel within（homemade 50 cm × 30 cm × 20 cm transparent plastic box） with cover,give 2% OVA atomized inhalation solution,build model;In the control group with normal saline instead.Results：The model group after antigen stimulate the mouth breathing,abdominal moved,irritability,restlessness,shaking,hair,nose,sneezing,very oral and ear purple,water quantity increased obviously,listlessness,eat less,unresponsive,activity significantly reduced or prone motionless,the colour yellow curly,such as asthma acute attack.Total number of white blood cells in BALF（bronchoalveolar lavage） and classification of eosinophil increased obviously.Nose and a small bronchus and lung tissue pathology slice see accompanying around blood vessels more inflammatory cells,numerous eosinophils cells infiltrating.Conclusion：The model of allergic rhinitis and asthma syndrome is established by the way in this study,which is comparable to the physiopathologic characteristics of human asthma.,the successful molding method has certain popularization value.