4种重金属胁迫对工业大麻种子萌发的影响

The Effects of 4 Heavy Metals Stress on Seed Germination of Industrial Hemp

通过发芽试验,研究重金属Zn、Pb、Cu、Cd胁迫对工业大麻种子萌发率、胚根伸长、胚芽生长以及总生物量的影响。结果显示,在4种重金属胁迫下,低浓度对种子萌发有促进作用,高浓度会抑制种子萌发和胚根的生长。4种重金属对种子萌发抑制作用依次为Cu2+和Cd2+>Zn2+>Pb2+,其中Zn2+和Pb2+在1 000 mg/L浓度时,萌发率仍然达到80%以上;对胚根长、胚芽长和总生物量的毒害或影响作用依次为Cu2+>Cd2+>Zn2+>Pb2+。结果表明,工业大麻种子萌发过程对Pb2+和Zn2+具有较高的抗性,抗性大小依次为Pb2+>Zn2+>Cd2+>Cu2+。

A germination test was conducted for understanding the effect of Zn , Pb, Cu and Cd on germination rate, radicle growth , bud development and total biomass of industrial hemp （ Cannabis sativa L.） , The results showed that 4 types of heavy metals with low concentration could promote seed germination , while heavy metals with high concentration would inhibit seed germination and radical growth.The toxicity of 4 types of heavy metals on seed germination rate could be ranked as Cu 2＋〉Cd2＋〉Zn2＋〉Pb2＋, and the germination rate could reach over 80 % for Zn2＋and Pb2＋with a concentration of 1 000 mg/L.The test also showed the same result for radicle growth, bud growth and total biomass and it could be ranked as Cu 2＋〉Cd2＋〉Zn2＋〉Pb2＋.This test indicated that seed germination of hemp was highly resistant to Pb 2＋and Zn2＋, and it could be ranked as Pb 2＋〉Zn2＋〉Cd2＋〉Cu2＋.