巨噬细胞炎性蛋白-1α在病毒性心肌炎患者血清中的变化及其与心肌损害的关系

Serum changes of macrophage inammatory protein-1α and its relationship with myocardial impairment in patients with viral myocarditis

目的 探讨巨噬细胞炎性蛋白-1α（MIP-1α）在病毒性心肌炎（VMC）患者血清中的变化及其临床意义.方法 收集26例急性期、20例恢复期VMC患者及25名健康人（对照组）血清,采用酶联免疫吸附法（ELISA）测定MIP-1α、心肌肌钙蛋白I（cTnI）水平,通过日立7180型全自动生化分析仪检测磷酸肌酸激酶同工酶（CK-MB）水平.结果 VMC急性期组血清MIP-1α、cTnI、CK-MB水平显著高于VMC恢复期组及对照组（P.＜0.01）;但VMC恢复期组与对照组比较,差异无统计学意义（P＞0.05）.VMC急性期患者MIP-1α与cTnI、CK-MB呈正相关（r=0.78,0.75;P＜0.05）;而VMC恢复期MIP-1α与cTnI、CK-MB无相关性（r=0.32,0.39;P＞0.05）.结论 MIP-1α可能在VMC的发生发展中起重要作用,可作为评价VMC患者心肌损害的血清学指标.

Objective To explore the changes of serum macrophage inammatory protein-la （MIP-la） levels in patients with viral myocarditis （VMC） and its clinical significance. Methods The serum was collected from 26 cases with the acute stage VMC, 20 cases with the recovery stage, and 25 healthy people（control group）. Serum levels of MIP-la and cardiac troponin I （cTnI） were measured using enzyme linked immunosorbent assay （ELISA）. Also, serum creatine kinase isozyme （CK-MB） levels were detected by the 7180 type holo-automatic biochemistry analyzer of Hitachi. Results Serum MIP-la, cTnI and CK-MB levels were higher in VMC group with the acute stage than those of VMC group with recovery stage and control group（P〈0.01 ）. There was no signif- icant difference in above indicators between VMC group with recovery stage and control group（P〉0.05）. There was positive correlations between MIP-la and cTnI as well as CK-MB （r=0.78, 0.75, P〈0.05） in VMC patients with acute stage, but these correlations did not exist in VMC patients with recovery stage （r=0.32, 0.39, P〉0.05）. Conclusion MIP-la may play an important role in the occurrence and development of VMC, and it can act as a serum indicator to evaluate myocardial impairment in VMC patients.