改良构建兔心肌缺血再灌注模型的体会

Improvement in making ischemia and reperfusion model of rabbit＆#39;s heart

目的 探讨制作兔心肌缺血再灌注模型的改进方法.方法 20只新西兰大白兔,“六线六结”改良法开胸后,随机以“双线双结”法结扎兔冠状动脉左前降支（常规组,10只）和左室支（观察组,10只）30 min,松结实施再灌注2h后,取心测梗死面积.术前及再灌注2h后,检测血肌钙蛋白Ⅰ.结果 两组制模成功率均为70％;两组手术后肌钙蛋白Ⅰ较术前均明显升高,左室支结扎组升高更明显;观察组造成的心肌梗死面积明显大于常规组（P＜0.05）,且变异小.结论 改良法可有效地提高兔心肌缺血再灌注模型构建的成功率.

Objective The research is aimed at improving methods in making of heart isehemic/reperfu- sion model of rabbit. Methods 20 New Zealand rabbits were randondy allocated to 2 groups （n-10） followed the procedure of ＂6 sutures and 6 knots＂ method to open the chest. Left anterior descending artery （LAD, control group） or left ventrieular artery （LVA, study group） were occluded for 30 rain using the ＂2 sutures and 2 knots＂ method, respectively. The hearts were enucleated after 120 rain reperfusion, and myocardial infarct size were de- termined. Bloods were drawn from femoral vein to evaluate cardiac troponin I （cTnI） values before operation and 120 rain after reperfusion in both groups. Results Incidence of pneumothorax and hemorthea were significantly re- duced during operation in this research, and achievement ratios were 70% in the 2 groups. There was an obvious elevation of cTnI in blood after operation（control group： 0.37＋0.12 vs 2.31±1.59, P〈0.05. Study group： 0.32＋0.14 vs 3.49±0.74, P〈0.01）. The infarct size in LVA occlusion group was bigger than that of LAD group, with less variation furthermore （control group： 21.51±7.87 %. Study group： 28.95±6.59%, P〈0.05）. Conclusion The new methods would improve achievement ratio of establishing ischemic/reperfusion model in rabbit＇s heart.