桑叶粉对链脲佐菌素糖尿病模型大鼠血糖及抗氧化功能的影响

Effects of Mulberry Leaf Powder on Blood Glucose and Antioxidant Role in Streptozotocin-diabetic Rats

目的:探讨桑叶粉(mulberry leaf powder,MLP)对链脲佐菌素(Streptozocin,STZ)糖尿病模型大鼠血糖及肝脏抗氧化功能的影响。方法:将STZ糖尿病模型组大鼠,按体重和血糖随机分为4组,每天分别灌胃给予蒸馏水(模型对照组),MLP低、中、高3个剂量组(250mg/kg、500mg/kg、1g/kg);另设阴性对照组和阴性MLP高剂量组,连续6w。实验结束后,模型组大鼠进行葡萄糖耐量实验(Oral Glucose Tolerance Test,OGTT)。留取模型对照组、MLP高剂量组和阴性对照组大鼠的肝脏,测定谷胱甘肽(GSH)活性及丙二醛(MDA)含量。结果:MLP高剂量组的摄食量和饮水量均低于模型对照组(P<0.05),但高于阴性对照组和阴性MLP高剂量组(P<0.05);血糖低于MLP中、低剂量组和模型对照组(P<0.05);其OGTT曲线下面积也较MLP中、低剂量组和模型对照组小,但差异无显著性(P>0.05);GSH活性低于阴性对照组(P<0.05);MDA含量低于模型对照组(P<0.05)。结论:桑叶粉能一定程度改善糖尿病大鼠多饮多食的症状,起到辅助降血糖的作用,对糖尿病大鼠的氧化应激有一定的减轻作用。

[Objective] To investigate the effects of mulberry leaf powder （MLP） on blood glucose and antioxidan! role in streplozotocin-diabetic rats. [Method ] Streptozotocin-diabetic rats were divided into 4 groups, and administered orally with distilled water, MLP at dose of 250, 500, 1 000mg/ （ kg · d） respectively, with one negative control group and one high dose mulberry leaf powder of negative group, respectively for 6 weeks. Diabetes groups were taken Oral Glucose Tolerance Test （OGTF） at the 6th weekend. The livers of diabetes group, high dose MLP of diabetes group, and control group were taken to measure glutathione （GSH） and malondialdehyde （MDA） vitality in the end of experiments. [Result] In high dose MLP of diabetes group, the food and water intake were both significantly less than diabetes group, but more than negative control group and high dose MLP of negative group. The blood glucose was lower than diabetes group, middle dose and low dose MLP of diabetes group. The area under OGTI＂ arcuation was less than diabetes group, middle dose and low dose MLP of diabetes group. The GSH vitality decreased obviously compared with control group. The MDA content was significantly less than diabetes group. [Conclusion] To some degree, the mulberry leaf powder could alleviate the symptom of polydipsia and polyphagia, and assist in decreasing blood glucose and relieve oxidative stress of diabetes rats.