期刊文献+

重组β-环糊精葡萄糖基转移酶生产β-环糊精的工艺条件优化 被引量:7

Optimization of β-cyclodextrin Production by Recombinant β-cyclodextrin Glycosyltransferase
下载PDF
导出
摘要 将来自于Bacillus circulans 251的β-CGTase编码基因克隆到表达载体pET-20b(+),转化Escherichia coli BL21(DE3)。经酶活检测培养基上清中的β-CGTase酶活为20 U/mL。对酶转化淀粉生成β-环糊精的反应条件进行了优化,结果表明,当底物马铃薯淀粉浓度15%,反应初始pH5.5,温度30℃,加酶量10 U/g干淀粉,环己烷浓度2.5%-5%(V/V),转化周期24 h,β-环糊精转化率达到最高值75.3%,是国内外报道的酶法生产β-环糊精的最高水平。 The βcgt gene encodingβ-CGTase from Bacillus circulans strain 251 was cloned into the expression vector pET-20b(+). The vector was then transformed into Escherichia coli BL21(DE3)for extracellular production ofβ-CGTase. The activity in the supernatant of recombinant E. coli BL21(DE3)was 20 U/mL. Furthermore, the condition forβ-cyclodextrin(β-CD)preparation by this recombinantβ-CGTase was optimized. At 15%potato starch, pH5.5, 30℃, 2.5%-5%(V/V)cyclohexane, 10 Uβ-CGTase per gram substrate incubated for 24 hours, 75.3%of the starch was transformed intoβ-CD. This is the highest level ofβ-CD conversion by enzyme method at home and abroad.
出处 《生物技术通报》 CAS CSCD 北大核心 2014年第8期175-181,共7页 Biotechnology Bulletin
基金 国家自然科学基金项目(31100048)
关键词 环糊精葡萄糖基转移酶 Β-环糊精 酶转化 重组表达 淀粉 Cyclodextrin glycosyltransferase β-cyclodextrin Enzymatic conversion Recombinant expression Starch
  • 相关文献

参考文献29

  • 1Martin Del Valle EM. Cyclodextrins and their uses : a review [ J ] . Process Biochemistry, 2004, 39 ( 9 ) : 1033-1046.
  • 2汪家铭.环状糊精生产应用及市场前景[J].武汉化工,1998(2):41-42. 被引量:2
  • 3田辉,杨国武,徐颐玲,谢伯泰.环状糊精与环状糊精葡萄糖基转移酶[J].工业微生物,1995,25(2):33-38. 被引量:16
  • 4Leemhuis H, Kelly RM, Dijkhuizen L. Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications [ J ] . Appl Mierobiol Biot, 2010, 85 ( 4 ) : 823-835.
  • 5Choi MJ, Ruktanonchai U, Min SG, et al. Physical characteristics of fish oil encapsulated by β-cyclodextrin using an aggregation method or polycaprolactone using an emulsion-diffusion method [ J ] . Food Chemistry, 2010, 119 : 1694-1703.
  • 6Shoo D, Sheng G, Chen C, et al. Removal of polychlorinated biphen- yls from aqueous solutions using β-cyclodextrln grafted multiwalled carbon nanotubes [ J ] . Chemosphere, 2010, 79 : 679-685.
  • 7王雁萍,谈重芳,梁秋霞,陈林海,秦广雍,霍裕平.应用02-5-71菌株葡萄糖基转移酶制备β-环糊精的研究[J].郑州工程学院学报,2003,24(4):52-55. 被引量:2
  • 8Biwer A, Antranikian G, Heinzle E. Enzymatic production of cyclodextrins [ J ] . Appl Microbiol Biotechnol, 2002, 59 : 609-617.
  • 9Pishtiyski I, Zhekova B. Effect of different substrates and theirpreliminary treatment on cyclodextrin production [ J ] . World Journal of Microbiology & Biateehnology, 2006, 22 : 109-114.
  • 10Penninga D, van der Veen BA, et al. The raw starch binding domain of eyclodextrin glycosyltransferase from Bacillus circular: strain 251 [ J ] . J Biol Chem, 1996, 271 ( 51 ) : 32777-32784.

二级参考文献17

  • 1张心平,张善稿.环状糊精葡萄糖基转移酶产生菌的初步鉴定及其产酶条件[J].南开大学学报(自然科学版),1993,7(2):63-68. 被引量:7
  • 2赵新帅,王占坤,祁庆生.环糊精糖基转移酶产物专一性改造:难题与挑战[J].生物工程学报,2007,23(2):181-188. 被引量:3
  • 3朱伯儒,史作清,何炳林.环糊精葡萄糖基转移酶的性质、应用与固定化研究进展[J].氨基酸和生物资源,1997,19(1):36-39. 被引量:6
  • 4Welliver M, McDonough J. Anesthetic related advances with cyclodextrins [J]. Scientific World,2007,7: 364 - 371.
  • 5Koyama J, Morita I, Kobayashi N. Simultaneous determination of anthra- quinones in rhubarb by high - performance liquid chromatography and capillary electrophoresis[ J]. Chromatogr A, 2007,1145 : 183 - 189.
  • 6Vogel R, Tandler B, Haussler L. Melt spinning of poly( 3 - hydroxybu- tyrate) fibers for tissue engineering using alpha- cyclodextrin/polymer inclusion complexes as the nucleation agent [ J ]. Macromol Biosci, 2006,6 (9) : 730 - 736.
  • 7Singh M, Sharma R, Banerjee U C. Biotechnological applications of cyclodextrins[J]. Biotechnol Advances,2002,20(5 - 6) : 341 - 359.
  • 8Nakagawa Y, Takada M. Site - directed mutations in Alanine 223 and Glycine 255 in the acceptor site of gamma - Cyclodextrin glucanotransferase from Alkalophilic Bacillus clarkii 7364 affect cyclodextrin production [ J ]. Biochem (Tokyo) ,2006,140(3) : 329 - 336.
  • 9淡家林 徐纯锡 任永娥.软腐芽孢杆菌D20产环糊精葡糖基转移酶的条件和酶性质.微生物学报,1988,28(2):136-142.
  • 10Bradfont MM. A rapid and sensitive method for the quanfitation of microgram quantities of protein utilizing the principle of protein - dye binding[ J ]. Analytical Biochemistry, 1976, 72(1-2):248-254.

共引文献19

同被引文献41

  • 1林勤保,杨连生,罗发兴,张本山.以淀粉为原料制造难消化糊精[J].食品与发酵工业,1995,21(5):49-51. 被引量:17
  • 2肖朝庭,傅衍,田勇.酿酒酵母细胞表面工程应用研究新进展[J].微生物学报,2005,45(5):812-816. 被引量:5
  • 3谢虹,周元元,夏小伟,梁建生.法夫酵母诱变选育高产虾青素菌株的研究[J].扬州大学学报(农业与生命科学版),2006,27(4):80-84. 被引量:3
  • 4汪红,祁玉峰,魏红.酶重量法测定食品中膳食纤维含量方法的改进[J].食品工业科技,2007,28(9):203-205. 被引量:21
  • 5Cheng J,Wu D,Chen S,et al.High-level extracellular production of α-cyclodextrin glycosyltransferase with recombinant Escherichia coli BL21 (DE3)[J].Journal of Agricultural and Food Chemistry,2011,59(8):3 797-3 802.
  • 6Ding R,Li Z,Chen S,et al.Enhanced secretion of recombinant α-cyclodextrin glucosyltransferase from E.coli by medium additives[J].Process Biochemistry,2010,45(6):880-886.
  • 7Tesfai B T,Wu D,Chen S,et al.Strategies for enhancing extracellular secretion of recombinant cyclodextrin glucanotransferase in E.coli[J].Applied Biochemistry and Biotechnology,2012,167(4):897-908.
  • 8Jacob RA, Sotoudeh G. Vitamin C function and status in chronic disease. Nutrition in Clinical Care: An Official Publication of Tufts University, 2002, 5 ( 2 ) : 66-74.
  • 9Naidu KA. Vitamin C in human health and disease is still a mystery? an overview. Nutrition Journal, 2003, 2( 1 ) : 7.
  • 10Yamamoto I, Muto N, Murakami K, Suga S, Yamaguchi H. L-ascorbic acid ct-glucoside formed by regioselective transglucosylation with rat intestinal and rice seed ct- glucosidases: its improved stability and structure determination. Chemical and Pharmaceutical Bulletin, 1990, 38(11): 3020-3023.

引证文献7

二级引证文献25

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部