7个柽柳ThMYB基因的鉴定及表达

Identification and Salinity-alkali Stress Expression Analysis of Seven ThMYB Gene from Tamarix hispida

通过对盐胁迫不同时间点柽柳根和叶组织的7个转录组数据进行分析，鉴定了7条MYB基因（命名为ThMYB1～ThMYB7），这些基因编码蛋白氨基酸残基数为206～918 aa，编码蛋白的相对分子质量为22．9～75．9，理论等电点为5．06～9．71。进一步分析了7个ThMYB基因在盐碱胁迫（0．3 mol·L-1NaHCO3）不同时间点柽柳不同组织中的表达谱，结果表明：尽管这7个ThMYB蛋白长度差异较大、同源性较低，但除ThMYB7外的其他7个ThMYB基因的表达均受盐碱胁迫调控，能对盐碱胁迫作出应答。

Seven full length cDNAs of ThMYB （ named as ThMYB1-7） were cloned from seven transcriptome cDNA libraries of Tamarix hispida.These ThMYB genes encode proteins of 206-918 amino acid residues with the molecular mass of 22.9-75.9 kDa and theoretical isoelectric point of 5.06-9.71.The gene expression profiles in T.hispida responding to NaHCO 3 were further investigated by analyzing seven transcriptome data .All ThMYB （ excepted ThMYB7） expression were regulated by saline-alkali stress, although these ThMYB proteins shared different length and low homology .It suggested that the ThMYBs may involved in the saline-alkali stress tolerance of T.hispida.