摘要
为构建马铃薯茎尖玻璃化法超低温保存体系,以6个马铃薯品种为试验材料,研究了预培养时间、茎尖大小、玻璃化液、玻璃化液处理时间及恢复培养基对马铃薯存活率和再生率的影响。结果表明:茎尖大小为1mm左右时,预培养24h后,于PVS2玻璃化液中浸泡30 min,而后转入MS+0.5 mg·L-1 Zeatin+0.10mg·L-1 NAA+1.0mg·L-1 GA3培养基中培养,其成活率和再生率最高。
In order to build cryopreservation system of potato stem tip by vitrification solution,taking six potato varieties as test materials,the effect of pre-culture time,size of stem-apex,vitrification solution and its soaking time,recovery medium on survival rate and regeneration rate were studied. The results showed that when the size of stem-apex was 1 ram,survival rate and regeneration rate were the highest with PVS2 vitrification soaking for thirty minutes and then transfer to the medium of MS＋0. 5 mg. L-1 Zeatin＋0. 10 mg. L-1 NAA＋ 1.0 mg- L-1 GA3 after pre-cuhure 24 h.
出处
《黑龙江农业科学》
2014年第8期12-17,共6页
Heilongjiang Agricultural Sciences
关键词
马铃薯
玻璃化法
离体茎尖
超低温保存
potato
vitrification
in vitro shoot tips
cryopreservation
