山茶花翻译控制肿瘤蛋白CjTCTP基因的电子克隆及生物信息学分析

Electronic Cloning and Bioinformatics Analysis of CjTCTP Gene from Camellia japonica L.

采用电子克隆方法从山茶花的EST数据库中获得了一条TCTP基因,并命名为CjTCTP,使用生物信息学方法对该基因编码蛋白从氨基酸组成、理化性质、跨膜结构域、疏水性/亲水性、信号肽、亚细胞定位、高级结构及功能域和进化树等方面进行了预测和分析。结果表明:山茶花CjTCTP基因全长706bp,包含507bp的完整开放阅读框,编码168个氨基酸;编码蛋白含有TCTP1及TCTP2保守域,是TCTP superfamily家族;从信号肽的预测可知,该蛋白不存在信号肽,可能为可溶性蛋白;亚细胞定位显示其可能位于细胞质中;同源性分析表明,该蛋白序列与拟南芥、橡胶树、玉米等物种TCTP蛋白序列的相似度达80%以上。

A novel gene of the translationally controlled tumor protein was cloned in silico base on the ESTs database of （Tamellia japonica L. and named （Tj TCTP. Some characterizations of animo acids encoded by CiTCTP gene were analyzed by bioinformatics tool,include composition of animo acid sequence, physical and chemical properties, t ransmen＋ brane domain, hydrophobicity/hydrophilicity, signal peptide, subsellular localization, secondary and tertiary structure of protein plus the functional domain,and phylogentic tree. The results showed that the full-length of CjTCTP gene was 706 bp with a complete ORF which encoded 168 amino acids. And the encoded amino acids contained TCTPI and TCTP2 domains which belonged to TCTP superfamily. Furthermore,the encoded amino acids were possibly a soluble protein contained signal peptide,and it might located in the cytoplasm. The CjTCTP amino acid sequence is highly homologous（more than 80%）with TCTPs of other plants, such as Arabid, psis thaliana, Hevea brasiliensis and Zea may L.