RP-HPLC法测定黄芪及玉屏风颗粒中4种异黄酮类成分

Simultaneous determination of four isoflavonoids from Astragali Radix and Yupingfeng Granules by RP-HPLC

目的建立定量测定黄芪饮片及玉屏风颗粒中毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮和芒柄花素的方法。方法采用RP-HPLC法,Phenomenex Luna C18色谱柱(250 mm×4.6 mm,5μm);乙腈-0.1%磷酸水溶液为流动相梯度洗脱;体积流量1 mL/min;检测波长251 nm。结果毛蕊异黄酮苷在0.132～0.792μg、芒柄花苷在0.066～0.396μg、毛蕊异黄酮在0.07～0.42μg、芒柄花素在0.034～0.204μg范围内分别与峰面积积分值呈良好的线性关系;黄芪饮片各成分平均回收率分别为100.7%(RSD为2.4%)、100.0%(RSD为2.0%)、99.7%(RSD为2.0%)和101.0%(RSD为2.0%)。玉屏风颗粒中4个成分的平均回收率分别为99.8%(RSD为2.2%)、101.2%(RSD为2.1%)、100.6%(RSD为2.1%)和98.4%(RSD为1.9%)。结论黄芪与白术和防风混合煎煮提取,可能提高了黄芪中毛蕊异黄酮苷和芒柄花苷两个成分的溶出率。

AIM To establish a method for determining four isoflavonoids （calycosin-7-glucoside, ononin, calycosin, and pureonebio） from Astragali Radix and Yupingfeng Granules. METHODS The quantitative analy- sis was carried out on a column of Phenomenex Luna C18 using a mobile phase of acetonitrile-0. 1% formic acid in a gradient elution mode at a flow of 1.0 mL/min. The detection wavelength was set at 251 nm. RESULTS The linear ranges were in the range of 0. 132 - 0. 792 μg for calycosin-7-glucoside, 0. 076 - 0. 456 μg for ononin, 0. 07 - 0. 42 μg for calycosin, and 0. 034 - 0. 204 μg for formononetin , respectively. The average recoveries of Astragali Radix were 100.7% （RSD=2.4%）, 100.0% （RSD=2.0%）, 99. 7% （RSD=2.0%）, 101.0% （ RSD = 2.0% ）, respectively. The average recoveries of Yupingfeng Granules were 99.8% （ RSD = 2.2% ）, 101.2% （RSD = 2. 1% ）, 100. 63% （RSD = 2. 1% ） and 98.44% （RSD = 1.9% ）, respectively. CONCLU- SION The co-decoction of Astragali Radix and Atractylodis macrocephalae Rhizoma and Saposhnikoviae Radix may elevate the resolution of calycosin-7-glucoside and ononin in Yupingfeng Granules.