杨梅核仁抗氧化肽的制备

Research on Preparation of Myrica rubra Nucleolus Antioxidant Peptide

利用碱性蛋白酶对杨梅核仁进行水解,制备得到生物活性肽。以·OH清除率为指标,研究了反应起始pH、酶用量、温度和酶解时间对制备杨梅核仁多肽工艺的影响;并以·OH清除率为指标,通过正交试验最终确定酶解杨梅核仁蛋白制备杨梅核仁多肽的工艺条件为:pH 10,酶用量1.3%,温度30℃,时间2 h。该条件下制备的杨梅核仁多肽·OH清除率为46.75%。

Obtain bioactive peptides was studied by alkaline proteinase hydrolysis from nucleolus ofMyrica rubra. The influence of substmte hydrolysis time, the initial reaction pH, enzyme dosage, temperature and enzymatic hydrolysis time on the preparation of protein from nucleolus ofMyrica rubra was researched. The process conditions of enzymatic hydrolysis of protein from nucleolus ofMyrica rubra were optimalized by orthogonal test. The optimum conditions were pH 10, enzyme dosagel.3%, 30 ~C, hydrolysis time 2 h. The .OH clearance rate of the hydrolysis ofMyrica rubra Nucleolus seed polypeptide under the condition is 46.75%.