富硒食用菌硒蛋白清除氧自由基作用研究

Studies on Scavenging Free Radicals by Selenoprotein of Enriched Edible Fungi

研究富硒食用菌硒蛋白对羟自由基(·OH)和超氧阴离子自由基(O2-·)的清除和抑制作用,为富硒食用菌的开发利用提供依据。利用Fenton体系产生·OH,邻苯三酚自氧化体系产生O2-·。以缓冲溶液为提取剂从富硒食用菌中提取硒蛋白,ICP-MS法和考马斯亮蓝染色法测定硒蛋白含量。结果表明,随着硒蛋白浓度的增大,对羟自由基的清除率随之增加,空白蛋白和无机硒对·OH的清除率之和小于富硒食用菌蛋白对·OH的清除率,且存在极显著差异。无机硒、普通食用菌蛋白和硒蛋白对超氧阴离子(O2-·)均有清除作用,随着体积的增加,三者对O2-·的清除率呈上升趋势,在不同浓度下,富硒食用菌蛋白对O2-·的清除率均高于普通食用菌蛋白以及无机硒。硒与食用菌蛋白之间在清除自由基方面存在协同作用。硒蛋白对O2-·有一定的清除和抑制作用。硒蛋白对·OH的清除效果好于对O2-·的清除效果。

This paper studied the selenium-rich edible fungi selenoproteins on hydroxyl free radical （·OH） and superoxide anion free radical （O2^-·） removal and inhibition,for selenium-rich edible fungi development provide the basis for the use;1,2,3-benzentriol antoxidation systems and Fenton reaction were used to observe the effects of selenoprotein on superoxide anion and hydroxyl radical scavenging and inhibition.The content of protein and selenium in Se-protein were measured by Coomassie Brillant Blue G-250 Dye Binding and ICP-MS;The result showed that along with the increasing concentration of selenoproteins, hydroxyl radical scavenging rate increases. Controlled protein and inorganic selenium on ·OH removal rate were less than the selenium-rich edible fungi protein on ·OH removal rate, and there was significant difference. Inorganic selenium, common edible fungus protein and selenium on superoxide anion （O2^-·） have clear role. As the size increases, the three on the O2^-· removal rate shows ascendant trend.Under different concentrations, selenium-rich edible fungi protein on O2^-· removal rate were higher than those of common edible fungus protein and inorganic selenium.Selenium and edible fungus protein in scavenging free radicals have synergistic effect. Effect of selenium on superoxide anion free radical scavenging and inhibition of a certain role. Selenoprotein on hydroxyl radical scavenging effect was better than the one on O2^-·.