HPLC-MS/MS法检测家禽鸡可食组织中阿奇霉素的残留量

Determination of Azithromycin's Residual Quantity in Poultry Chicken Edible Organizations by HPLC-MS/MS

建立了检测家禽鸡可食组织中阿奇霉素残留量的HPLC-MS/MS法,主要色谱条件为:XDB-C18色谱柱(1.8μm,2.1×30mm);以0.1%甲酸的水和甲醇(60∶40,体积比)作为流动相;流量为0.4mL/min;以MRM扫描模式,母离子m/z 749.5,定量离子m/z 589.5,定性离子m/z 157.8进行检测。结果表明:该方法加标线性范围为0.01μg/mL^0.20μg/mL与0.20μg/mL^1.0μg/mL相关系数均大于0.990,出峰时间为1.231 min,最低检测限为0.001μg/mL,采用本法测定鸡可食组织中阿奇霉素回收率在73.6%以上,日内日间变异系数均小于5%。

The purpose was to establish a method of determination azithromycin＇s residual quantity in poultry chicken by HPLC-MS/MS. The samples were separated on XDB-C18 column （1.8μm,2.1×30 mm）, eluted with mixture of water with 0.1%methanoic acid and methanol（60∶40,volume ratio）at 0.4 mL/min, scan by MRM with parent ion m/z 749.5, quantify ion m/z 589.5 and determin ion m/z 157.8. Results showed that the calibration curces of standard test had good linearity over the range from 0.01μg/mL-0.20μg/mL and from 0.20μg/mL-1.0 μg/mL with a correlation coefficient of 0.990. The minimum detection limit was 0.001μg/mL and the peak time was 1.231min. By adopting the method for the determination of chicken in edible tissues of azithromycin recovery rate is above 73.6%, within-day and between-day coefficients of variation were less than 5%.