超临界CO_2流体萃取法提取迷迭香中主要挥发性成分研究

Extraction of Rosemary Oil by Supercritical Carbon Dioxide Fluid and Activity Component of the Extract

以云南迷迭香为原料,利用超临界CO2流体萃取装置提取迷迭香精油,然后采用GC-MS联用分析仪对所得的萃取物进行分析。通过单因素和正交试验优化萃取条件,实验结果表明萃取温度40℃、萃取压力20 MPa、萃取时间120 min、CO2流量25L/h为最优萃取条件。通过GC-MS联用进行活性成分分析,所得迷迭香精油主要是樟脑(25%),桉叶油素(32%),乙酸铵(9%),beta.-蒎烯(4%)等混合香气构成了迷迭香的特征香气。

Essential oil was extracted from the stems and leaves of the plant produced in Yunnan by Supercritical Carbon Dioxide Fluid Extraction. With the single-factor and orthogonal design, to determine the optimal conditions test. Experimental results show that the optimal conditions as follow：Extraction temperature 40℃, Pressure 20 MPa, Time 120 min, CO2 Flow 25 L/h. The activity components of the essential oil were analyzed by GC-MS. From the Experimental date, we can see Essential oil composition is complex and diverse. Mixed aroma of high contents, such as Camphor （25%）,Eucalyptol （32%）,Ammonium acetat e （9%）,beta.-Pinene （4%）, make up the featured aroma of Rosemary.