利用多重PCR-RFLP同时检测羊肉及掺杂其中的肉类

Using Duplex PCR-RFLP Technology to Detect Mutton and Other Meat Adulteration

以纯羊肉为原料,按2%、5%和10%的重量比在羊肉中掺杂鸡肉、猪肉和鼠肉,通过基因组DNA提取试剂盒法提取DNA,利用多重多聚酶链反应(Polymerase Chain Reaction,PCR)及限制性片段长度多态性(Restriction Fragment Length Polymorphism,RFLP)分析同时检测羊肉及掺杂其中的其他肉类。结果表明,通过多重PCR扩增,可以有效的检测出纯羊肉及其掺杂的鸡肉、猪肉和鼠肉,检出限为2%。

Pure mutton or the mixture with 2%, 5%and 10%chicken, pork and mouse meat were studied. Genomic DNA was extracted with t DNA extraction kit. Pure mutton and the mixtures were detected simultaneously by duplex polymerase chain reaction（PCR） and restriction fragment length polymorphism（RFLP） techniques. The results showed that method introduced in this study could detect pure mutton , chicken, pork or mouse meat adulterated in it effectively. The detection limitation was 2%.