鳢肠不同部位抗骨质疏松活性及化学成分比较研究

Comparative Study on Antiosteoporosis Activity and Chemical Composition from Different parts of Eclipta prostrata L.

为了比较鳢肠不同部位提取物的抗骨质疏松活性及总皂苷、总黄酮含量,采用MTT法和对硝基苯酚磷酸二钠法测定鳢肠不同部位乙醇提取物对UMR106细胞的增殖和碱性磷酸酶(ALP)活性;采用紫外分光光度法测定鳢肠不同部位总皂苷和总黄酮含量。结果表明,鳢肠根和叶提取物对UMR106细胞的增殖具有促进作用,浓度为50μg/mL时促增殖作用最强,增值率分别为7.7%和16.9%,茎提取物没有明显的作用;鳢肠根、茎、叶提取物对ALP活性均有促进作用,浓度为100μg/mL时促增殖作用最强,分别促进18.9%、20.1%和17.0%;鳢肠根、茎、叶中总皂苷含量分别为3.6%、4.0%和2.2%,总黄酮含量分别为1.4%、1.4%和3.4%。这些结果表明;鳢肠不同部位提取物均有抗骨质疏松活性,其不同部位总皂苷和总黄酮的含量差异较大。

To Compare the anti-osteoporosis activity and the contents of total saponins and total tlavonoids from different parts of Eclipta prostrata L.. The effects of ethanol extracts from different parts of E. prostrata on the proliferation and differentiation of UMR106 ceL were evaluated by the MTY method and measuring the activity of alkaline phosphatase （ALP） by p-nitrophenyl phosphate disodium method. The contents of total saponius and total ilavonoid from different parts of E. prostrata were used to determine by Ultraviolet spectrophotometry method. The results show that the extracts of the root and leaf from E. prostrata stimulated UMR106 cells proliferations,the proliferation rate was 7.7% and 16. 9% respectively at a concentration ofS0 p.g/mL,while the stem did not stimulate UMR106 cells proliferation, all of the extracts from root, stem and leaf of E. prostrata could increased the ALP activity respectively by 18.9% ,20.1%, and 17.0% at a concentration of 100 μg/mL. The contents of total saponin and total flavonoids from the root,stem and leaf of E. prostrata were 3.6% ,4.0% ,2.2% and 1.4%, 1.4% ,3.4%, respectively. In Conclusion, all of the extracts from different parts of E. Prostrata have antiostcoporotic activity. The contents of total saponius and total flavonoids from different parts of E. prostrata have large difference.