木醋液的植物生长调节剂特性的分子机理研究

Study on Molecular Mechanism of Wood Vinegar's Plant Growth Regulator-like Character

木醋液(wood vinegar,WV)是一种农业生产上广泛应用的植物生长调节剂类似物,但对其分子水平调节机制研究的缺乏严重限制了它的更进一步应用。试验以模式生物拟南芥为材料,通过半定量PCR(Semi-quantitative PCR,SQ-PCR)和条件控制培养,研究了不同浓度、不同时间的木醋液、生长素吲哚乙酸(IAA)处理对生长素诱导基因表达和形态学变化的影响。结果表明,木醋液可调节拟南芥生长素诱导基因Aux/IAA1、Aux/IAA5、Aux/IAA19、ARF19和SAUR-AC1的表达。通过Aux/IAA和ARF蛋白相互作用,木醋液调节途径中存在与生长素类似的负反馈。在形态学方面,木醋液、生长素IAA均可抑制叶片数量和叶片伸展,促进主根伸长和侧根形成。这表明木醋液不但以与生长素IAA相似的途径促进植物生长,且以自身的调节方式促进植物生长发育。

Woed vinegar （WV） is an important plant growth regulator-like substance used in agriculture worldwide, but little known on its mechanism at molecular level restricted its wider and further application. In this study, the effects of WV and auxin on the growth ofArabidopsis thaliana were compared at both genetic and morphologic level through semi- quantitative PCR （SQ-PCR） and condition-contrelled culturing. The results showed that WV can regulate auxin-induc- ible genes like Aux/IAA1 ,Aux/IAA5 ,Aux/IAA19 ,ARF19 and SAUR-AGl expression. Through interaction between Aux/ IAAs and ARFs,WV had a negative feedback in its regulation pathway,similar to the homolog of auxin IAA＇ s. In addition,the morphologie comparison results showed that WV and auxin IAA both repressed leaf number and its expansion but promoted main root elongation and lateral root formation. These results indicated that the regulation effect of WV was similar but not identical to that of auxin. The results presented in this study will contribute to the wider and more precise usage of WV in agriculture.