摘要
目的观察消癥散瘀方对子宫内膜异位症造模后大鼠MAPK信号通路表达的影响,探讨其治疗内异症的作用机理。方法将48只SD大鼠随机分为正常对照组、内异症模型组、消癥散瘀方组、丹那唑组各12只,采用免疫组化检测各组大鼠异位内膜C-SRC、EFR、ERK的蛋白表达。结果与正常对照组比较,模型组异位内膜C-SRC、EFR、ERK蛋白表达明显升高(均P<0.01);与模型组比较,消癥散瘀方组异位内膜C-SRC、EFR、ERK蛋白表达明显降低(均P<0.01)。结论消癥散瘀方能够降调子宫内膜异位症模型大鼠MAPK信号通路,从而降低子宫内膜细胞侵袭、转移、种植的能力。
Objective To observe the effects of Xiao-Zheng-San-Yu-Fang on MAPK of EM model and to investigate its mechanisminthetreatmentofEM.Method 48ratswererandomlydividedintoacontrolgroup,amodelgroup,aXiao-Zheng-San-Yu-Fang group and a Danazol group, each consisting 12 rats.The positive expressions of C-SRC、EFR、ERK protein in the focal tissue were tested with immunohistochical method.Findings Compared with the normal control group, the positive expressions of C-SRC, EFR, and ERK protein in the focal tissue as observed in the model group were remarkably higher （ P〈0.01）;Compared with the model group, the positive expressions of C-SRC, EFR, ERK protein in the focal tissue as observed in the Xiao-Zheng-San-Yu-Fang group were remarkably lower（P〈0.01）.Conclusion Xiao-Zheng-San-Yu-Fang can decrease the positive expressions of C-SRC, EFR, and ERK protein in the focal tissue.Therefore, they can inhibit the development of heterotopic membrane and thus keep the cells from developing an ability to invade, transpose, and implant.
出处
《健康研究》
CAS
2014年第4期380-382,共3页
Health Research
基金
浙江省教育厅资助项目(Y201121064)
浙江省中医药科技计划项目(2012ZB065)
