延生胶囊的薄层色谱鉴别及二苯乙烯苷的含量测定

TLC Identification and HPLC Determination of 2,3,5,4'-tetrahydroxystil bene-2-O-β-D-glucosde inYansheng Capsules

目的建立延生胶囊的薄层色谱鉴别及二苯乙烯苷的含量测定方法。方法用薄层色谱法(TLC)对当归、独活、羌活、川芎、防风、麻黄、制何首乌进行鉴别;用高效液相色谱法(HPLC)测定二苯乙烯苷的含量。结果薄层鉴别斑点清晰;二苯乙烯苷平均加样回收率为98.3%,RSD=1.16%。结论该鉴别方法重现性好,专属性强;含量测定方法简便、准确,可用于延生胶囊的质量控制。

Objective To establish identification and quantitative determination method of Yansheng capules. Methods TLC was employed to identify six crude herbal elements in Yansheng capules and the content of 2,3,5,4＇-tetrahy- droxystil bene-2-O-β-D-glucosde was determined by HPLC. Results The spot developed on TLC was fairly clear. The average recovery of 2,3,5,4＇-tetrahydroxystil bene-2-O-β-D-glucosde was 98.3% with RSD1.16%. Conclusion The identification method is repeatable and specific, and the quantitative determination method is sim- ple and accurate, which can be used for the quality control of Yansheng capsules.