帕金森病患者脑脊液中miR-133b的表达及其临床意义

Expression of miR-133b and its clinical significance in cerebrospinal fluid of patients with Parkinson's disease

目的检测miR-133b在帕金森病(PD)患者脑脊液中的表达情况,为帕金森病的早期诊断提供参考依据。方法采用实时荧光定量RT-PCR检测46例PD患者、25例头痛患者和22例健康人脑脊液中miR-133b表达水平。分析脑脊液中miR-133b表达水平与PD相关临床指标的关系。结果 PD组患者脑脊液中miR-133b相对表达水平明显高于头痛组和健康对照组(P均<0.05),且PD患者脑脊液中miR-133b表达与帕金森综合评分量表(UPDRS)分级呈正相关(r=0.74,P<0.05)。ROC曲线确诊PD患者的脑脊液miR-133b临界值为0.672,其诊断敏感度为87.7%,特异度为79.3%。结论脑脊液miR-133b的检测不仅有助于PD的诊断,也可能作为判断PD病情的指标之一。

Objective It is to detect the expression of miR -133 b in cerebrospinal fluid of patients with Parkinson ’ s dis-ease （ PD） , so as to provide a reference on early diagnosis of PD .Methods The expression levels of miR -133 b in cerebro-spinal fluid of 46 patients with PD , 25 patients with headache and 22 healthy people were detected by Reverse transcription polymerase chain reaction （RT-PCR）.The correlations of miR-133b expression and the clinicopathological features of PD were analyzed.Results The expression level of miR -133b in cerebrospinal fluid of patients with PD was significantly higher than headache group and healthy control group （all P〈0.05）.The expression level of miR -133b in cerebrospinal fluid had a positive correlation with Unified Parkinson ’s Disease Rating Scale （UPDRS） （r=0.74, P〈0.05）.The critical values for miR-133b in cerebrospinal fluid were detected at 0.672 through the ROC curves.Under the critical value, the sensitivity of miR-133b was 87.7%and the specificity was 79.3%.Conclusion The detection of miR -133b in cerebrospinal fluid not only contributes to the diagnosis of PD , but also be as an index to judge the condition of PD .