微囊藻藻胆蛋白光敏杀虫剂毒杀机制研究

Toxicity mechanism of Microcystis phycobiliprotein as photosensitive insecticide

用果蝇(Drosophila melanogaster)和草地夜蛾卵巢细胞Spodoptea Frugiperda 21(SF21)来探讨微囊藻藻胆蛋白(MC-PBP)作为光敏杀虫剂的作用和机理。实验结果表明:MC-PBP对果蝇的LC50为750μg/mL,致死剂量为4000μg/mL;血卟啉甲醚(HMME)对果蝇的LC50为1000μg/mL。MC-PBP浓度4000μg/mL时,果蝇的死亡率是93.33%,而相同浓度下,HMME光敏剂的致死率是66.66%;在细胞水平研究中发现:MC-PBP光照处理SF21细胞48 h之后,400μg/mL的MC-PBP对细胞的抑制率达到65%;MC-PBP处理SF21细胞后,其细胞内丙二醛(MDA)含量明显升高,而细胞内还原型GSH相对含量随MC-PBP浓度升高而显著降低。结果表明MC-PBP光敏杀虫剂毒杀机制与细胞内脂质过氧化和细胞抗氧化损伤能力减弱相关。

The experiment studied the toxicity mechanism of Microcystis phycobiliprotein as photosensitive insecticide by Drosophila melanogaster and Spodoptea Frugiperda 21（ SF21）. The results showed that the LC50 and lethal dose of MC-PBP on Drosophila melanogaster were 750 μg /mL and 4000 μg /mL,respectively,but LC50 of hematoporphyrin monomethyl ether in the control group on Drosophila melanogaster was 1000 μg /mL. When the concentration of MC-PBP was 4000 μg /mL,the death rate of Drosophila melanogaster was 93. 33%,and under the same concentration,the death rate of hematoporphyrin monomethyl ether was 66. 66%. Cellular analysis indicated that MC-PBP by light processing after 48 hours,the inhibition rate of MC-PBP on SF21 was 65%; the relative MDA content of the SF21 cells significantly increased when treated with different concentrations. However,under the same conditions,the relative GSH content of the SF21 cells significantly decreased with increasing concentrations of MC-PBP compared to the control group.Results showed that toxicity mechanisms of Microcystis phycobiliprotein as photosensitive pesticide were related to intracellular lipid peroxidation and cell oxidative damage resistance.