α-淀粉酶协同超声波法提取复方半边莲注射液工艺优化

Optimization on Extraction Technology of Compound Lobelia Injection by Synergistic Action of α-amylase and Ultrasonic Method

在超声波条件固定的情况下,采用正交试验设计,运用α-淀粉酶提取复方半边莲注射液指标成分野黄芩苷,以HPLC方法检测野黄芩苷含量.最佳工艺条件为:酶解时间30 min,pH6.0,酶解温度30℃,酶用量0.5 g.在此优化条件下,提取复方半边莲注射液的野黄芩苷含量为4.45 g/L,明显大于复方半边莲注射液注册标准煎煮法野黄芩苷的含量1.34 g/L、单一超声法3.84 g/L、单一α-淀粉酶法1.89 g/L.

The scutellarin of compound lobelia injection was extracted by the intergrated treatment with α-amylase and ultrasonic, and was determined by HPLC in orthogonal experiment. The optimal conditions were the following： the enzymolysis time is 30 min, the optimal pH is 6.0,the cnzymolysis temperature is 30 % with the α-amylase dosage is 0.5 g . Under such optimized conditions, the content of scutellarin in compound lobelia injection is 4.45 g/L, which is significantly more than 1.34 g/L through decocting technology contained in registration standards ,3.84 g/L through single ultrasonic extraction, and 1.89 g/L trough single α-amylase extraction.