山西新记录Tripylina zhejiangensis记述

Description of Tripylina zhejiangensis(Nematoda: Enoplida:Trischistomatidae),a New Record from Shanxi Province

采用浅盘法分离采集太谷自由生活的线虫，经分离并脱水制片后，用微分干涉显微镜进行形态观察和数值测量，并结合LSU和SSU的序列比对鉴定该线虫。结果表明，其雌虫形态特征为热杀死后向腹部弯曲，头部6根长刚毛和4根短刚毛位于同一圈，背部牙齿位于亚腹部牙齿的前端，背部牙齿和亚腹部牙齿形状均呈三角形，大小相似；在亚腹中部有颈刚毛1根；无后阴子宫囊；尾部有1对刚毛；雌虫体长924-1204μm，a为22—28，b为5．0—6．0，c为16．2～21．2；c’为2．0—2．7；V为65％～67％；雄虫未发现。将其LSU和SSU序列测序后与NCBI中已有的Tripylinazhejiangensis序列比对，相似率分别达100％和99％。根据其形态学和序列特征将其鉴定为Tripylina zhejiangensis，此种为山西省首次报道。

The morphological and molecular characters were used to identify the nematodes found in Taigu county, Shanxi province, China. The nematode is characterized by having a triangular dorsal tooth, pointing towards the ventral side, anterior to two sub-ventral teeth, with dorsal and sub-ventral teeth similar in shape and size; one ventromedian setae in the cervical region; absence of post-uterine sac; and a pair of setae on the tail; female body length 924-1 204 μm, a = 22-28, b = 5.0-6.0, c = 16.2-21.2, c＇ = 2.0-2.7 and V = 65%-67%; male not found. When compared the molecular data of present species with the published sequences in the GenBank, there are 100% nucleotide similarity for D2D3 region of LSU and 99% nucleotide similarity for SSU with Tripylina zhejiangensis. So the nematode was identified as T. zhejiangensis and it was the first time to be reported in Shanxi province, China.