原创中药红珠胶囊基于RRLC-ESI-MS/MS技术的化学成分分析

Chemical Component Analysis of Hongzhu Capsule,a New Original Chinese Medicine,by RRLC-ESI-MS/MS

采用快速液相-三重串联四极杆质谱(RRLC-ESI-MS/MS)技术,在电喷雾质谱正模式和负模式下,对红珠胶囊内容物进行分析鉴定。通过质谱中分子离子峰和碎片离子峰的精确相对分子质量匹配,保留时间,紫外光谱对比,与对照品比对并结合参考文献鉴定,确证和指认了红珠胶囊中的新北美圣草苷、樱桃苷、柚皮苷、忍冬苷、野漆树苷、橙皮内酯水合物、7-(2″-α-鼠李糖基-6″-(3″″-羟基-3″″-甲基戊二酰)-β-D-葡萄糖基)柚皮素、异橙皮内酯、柚皮素9个化学成分。研究揭示了红珠胶囊的化学物质基础,为控制该药质量提供了依据。

RRLC-ESI-MS/MS technique was applied to identify the components of Hongzhu Capsule . ESI-MS/MS analysis was then conducted under both positive and negative modes , matching molecular ion peak , fragment ion peak , retention time and DAD .Nine chemical components , including Neoeriocitrin , Prunin, Naringin, Lonicerin, Rhoifolin, Meranzin hydrate, Meliditin, Isomeranzin, Naringenin were i-dentified .The findings provided the basis for the quality control and pharmacodynamic constituents of Hongzhu capsule .