摘要
We establish a system to measure the functional absorption cross section of photosystem lI （PSII） （O-PSII） and maximum quantum yield of photochemistry in PSII （Fv/Fm）. The system utilizes a sequence of high-frequency excitation flashes at microsecond intervals to induce a microsecond-level fluorescence yield curve. Parameters o-Psii and Fv/Fm are calculated by fitting the curve using nonlinear regression. Experimental results show that the relative standard deviation （RSD） of the system is less than 3%, and the correlation coefficient of Fv/Fm values measured by this system and those measured by pulse amplitude modulation method is 0.950.
We establish a system to measure the functional absorption cross section of photosystem lI （PSII） （O-PSII） and maximum quantum yield of photochemistry in PSII （Fv/Fm）. The system utilizes a sequence of high-frequency excitation flashes at microsecond intervals to induce a microsecond-level fluorescence yield curve. Parameters o-Psii and Fv/Fm are calculated by fitting the curve using nonlinear regression. Experimental results show that the relative standard deviation （RSD） of the system is less than 3%, and the correlation coefficient of Fv/Fm values measured by this system and those measured by pulse amplitude modulation method is 0.950.
基金
the Natural Science Foundation of Anhui Province(No.1408085MD72)
the National"863"Program of China(Nos.2014AA06A509,2013AA065502,and 2009AA063005)
the Science and Technology Planning Project of Anhui Province(No.1206c0805012)
the National Natural Science Foundationof China(No.61378041)
the Excellent Youth Foundation of Anhui Scientific Committee(No.1108085J19).
