摘要
目的研究非编码小RNA piRNA-651对骨肉瘤U20S细胞增殖、侵袭与转移的影响。方法通过RNA干扰技术沉默U20S细胞中的piRNA-651,并应用实时定量PCR(qRT-PCR)检测骨肉瘤U20S细胞中piRNA-651的表达情况;流式细胞术检测细胞周期的变化,MTT实验检测沉默piRNA-651表达对细胞增殖的影响,Transwell侵袭实验检测细胞侵袭能力的变化,细胞划痕实验检测细胞转移能力的变化。结果 piRNA-651-siRNA可有效沉默piRNA-651在骨肉瘤细胞U20S的表达,敲低U20S细胞中piRNA-651的表达可以明显阻滞骨肉瘤细胞的细胞周期,抑制骨肉瘤细胞的增殖、侵袭与转移能力。结论 piRNA-651在骨肉瘤的发生发展及侵袭转移中发挥着重要作用。
Objective To investigate the effects of piRNA-651 on the proliferation,invasion and migration of human osteosarcoma cancer U20S cells.Methods The piRNA-651 mRNA expression of U20S cell U20S transfecting was detected with siRNA or control siRNA by quantitative real time PCR （qRT-PCR）.After siRNA and control siRNA transfected into U20S cells for 48 hours.The proliferation of U20S cells were analyzed by MTT.The cell cycle of U20S cells was analyzed by flow cytometry,U20S cells invasion ability was analyzed by Transwell cell invasion assay.The migration capacity of U20S cells was detected by wound healing assay.Results The mRNA expression level of piRNA-651 in U20S cells was effectively silenced by piRNA-651-siRNA and U20S cells proliferation and cell cycle was inhibited after transfected piRNA-651-siRNA.The invasion and migration abilities of U20S cells decreased after transfected piRNA-651siRNA.Conclusion piRNA-651 may play an important role in the progression,invasion and migration of human osteosarcoma.
出处
《西部医学》
2014年第8期971-974,共4页
Medical Journal of West China
