白细胞介素-22对人表皮黑素细胞生物活性的影响

Effects of IL-22 on biological activity of human epidermal melanocytes

目的观察白细胞介素(IL)-22对黑素细胞生物活性的影响,包括黑素细胞的增生、黑素细胞酪氨酸酶活性和黑素细胞凋亡,从而研究色素障碍性皮肤病的发病机制,为色素性疾病的治疗提供新的思路。方法建立模拟正常生理状态的黑素细胞体外培养体系,用不同浓度IL-22作用于黑素细胞,采用CCK-8法检测不同浓度IL-22对黑素细胞增生活性的影响;L-DOPA法检测其对黑素细胞酪氨酸酶活性的影响;流式细胞仪检测其对黑素细胞凋亡的影响。结果 1 IL-22随着浓度的增加呈明显的时间依赖性抑制黑素细胞的增生,与空白对照组相比差异有统计学意义(P<0.05)。2用浓度为100 ng/ml的IL-22培养黑素细胞,48 h后细胞凋亡率为23.0%,空白对照组为5.1%。结论 IL-22随着浓度的增加明显抑制黑素细胞的增生,呈时间依赖性,当培养时间为72 h时,所有浓度均抑制黑素细胞的增生,黑素细胞酪氨酸酶活性也随着IL-22浓度的增加而降低,黑素细胞凋亡率较空白对照组明显升高,均提示IL-22可引起黑素细胞的损伤。

Objective To observe the effects of IL-22 on biological activity of human epidermal melanocytes, including proliferation, tyrosinase activity, and apoptosis of melanocytes. The results of the study would help us to better understand the pathogenesis of pigment aplastic skin diseases and provide new ideas for the treatment of pigment disorders. Methods An in vitro culture system of melanocytes simulated normal physiological state was established, then the effects of IL-22 of different concentrations on proliferative activity of human epidermal melanocytes were detected by CCK-8 method, meanwhile the effects on tyrosinase activity were detected by L-DOPA method, and the effects on apoptosis were detected by fl ow cytometry. Results With the increase of concentration, IL-22 exhibited time-dependent inhibitory effect on the proliferation of melanocytes, for the inhibitory ratio of melanocytes, there was signifi cant difference between the IL-22 groups and the control group（P〈0.05）. For the melanocytes cultured at the concentration of 100 ng/ml of IL-22 and the control group, the apoptosis rate of melanocytes were 23.0% and 5.1% respectively after 48 h of cell culture. Conclusions With the increase of concentration, IL-22 exhibited time-dependent inhibitory effect on the proliferation of melanocytes. When the culture time over 72 hours, the proliferation of melanocytes was inhibited in IL-22 groups with all the different concentrations; meanwhile with the increase of the concentration of IL-22, the tyrosinase activity of melanocytes gradually decreased; compared with the control group, the apoptosis rate of melanocytes in IL-22 groups increased signifi cantly. All these results suggest that IL-22 may cause damage of melanocytes.