转谷氨酰胺酶4在前列腺癌组织中的表达及意义

Expression of transglutaminase 4 in prostate cancer and its clinical significance

目的探讨转谷氨酰胺酶4(transglutaminase 4,TGM4)在前列腺癌诊断及预后评价中的可能价值。方法对159例前列腺癌患者的存档石蜡标本行TGM4免疫组织化学染色;根据染色情况将TGM4表达水平分为4级:不表达,弱表达,中表达和高表达。通过查阅病历、调用我院前列腺癌随访数据库和电话随访等方式获得患者临床病理资料及随访信息;进而对TGM4表达水平与前列腺癌临床病理特征及预后信息的关系进行统计学分析。结果与癌旁组织相比,TGM4在前列腺癌组织中高表达(P<0.001),且表达水平在不同Gleason分级(P<0.001)和前列腺特异抗原(PSA)水平(P=0.005)间差异有统计学意义。单因素Cox回归分析提示TGM4高表达是前列腺癌生化复发的高危因素(P=0.020),但多因素Cox回归分析并不支持TGM4高表达是前列腺癌生化复发的高危因素(P=0.139)。结论 TGM4在前列腺癌中高表达,且在高Gleason分级和高PSA水平的前列腺癌中表达更高,值得进一步研究。

Objective To investigate the value of transglutaminase 4 in diagnosis and prognosis prediction of prostate cancer.Methods Transglutaminase 4 immunostaining was performed with paraffin sections from 159 prostate cancer patients receiving radical prostatectomy,and the staining results were divided into 4 levels：negative staining,weak staining,moderate staining,and strong staining.The clinical and pathological information of the patients were obtained by reviewing the medical records.The follow-up data were collected by reviewing medical records,prostate cancer database of our department,and telephoning the patients or their family members.Expression of transglutaminase 4 and clinical,prognosis data of patients were subjected to statistical analysis.Results The expression of transglutaminase 4 in the prostatic cancer tissue was significantly higher than that in the adjacent normal tissue（P0.001）;and the expressions were significantly different in patients with different Gleason grades（P0.001）and different prostate specific antigen levels（P=0.005）.Univariate Cox regression analysis indicated that high transglutaminase 4 expression was a risk factor of biochemical recurrence of prostatic cancer（P=0.020）,but multivariate Cox regression analysis did not support this finding（P=0.139）.Conclusion Transglutaminase 4 expression is increased in prostate cancer tissues,and the expression is stronger in malignant tissues with higher Gleason grade and prostate specific antigen level.