绿原酸对草鱼鱼种生长、非特异性免疫和肉质的影响

THE EFFECTS OF CHLOROGENIC ACID ON THE GROWTH, NON-SPECIFIC IMMUNE INDEX AND THE MEAT QUALITY OF JUVENILE GRASS CARP(CTENOPHARYNGODON IDELLUS)

为考察绿原酸对草鱼鱼种生长、非特异性免疫指标和肌肉品质的影响,在基础饲料中分别添加0.00%(对照组)、0.01%、0.02%、0.04%、0.06%、0.08%绿原酸,饲养平均体重(3.19±0.03)g的草鱼鱼种。经60d饲养,各组草鱼增重率分别为283.1%、305.7%、307.3%、329.6%、308.3%、303.0%,饲料系数分别为1.45、1.33、1.33、1.26、1.30、1.36;与对照组相比,添加0.04%绿原酸显著提高草鱼鱼种增重率,降低了饲料系数(P<0.05),添加0.01%、0.02%、0.06%绿原酸有提高草鱼鱼种增重率的趋势;在饲料中添加0.04%绿原酸可显著提高肝胰脏SOD活性(P<0.05),添加0.08%绿原酸可显著降低血浆天门冬氨酸氨基转移酶活性(P<0.05),各组肝胰脏溶菌酶、血浆碱性磷酸酶和丙氨酸氨基转移酶活性无显著差异(P>0.05)。对肌肉成分的分析表明,各组水分、粗脂肪含量无显著差异(P>0.05),但添加0.04%绿原酸可显著提高肌肉粗蛋白、缬氨酸、异亮氨酸、组氨酸、必需氨基酸和总氨基酸含量(P<0.05)。添加0.02%绿原酸可显著提高肌肉羟脯氨酸、胶原蛋白含量(P<0.05)。综上所述,适量绿原酸能改善草鱼鱼种生长性能、非特异性免疫功能和肌肉品质。草鱼鱼种饲料中绿原酸添加量推荐为0.04%。

To investigate the effects of chlorogenic acid on the growth, non-specific immune function and the meat quality of juvenile grass carp, the diets were improved with the addition of chlorogenic acid at the concentrations of 0.00% （control group）, 0.01%, 0.02%, 0.04%, 0.06%, and 0.08%. Fish with an average weight of （3.19±0.03） g were selected and fed for 60 days. The results showed that the growth rate of the six diet groups were 283.1%, 305.7%, 307.3%, 329.6%, 308.3%, 303.0%, and the FCR was 1.45, 1.33, 1.33, 1.26, 1.30, and 1.36, respectively. The growth rate and FCR were significantly improved with the addition of 0.04% chlorogenic acid compared with the control group （P0.05）. In terms of the muscle composition and the meat quality, there were no significant differences in muscle moisture and crude lipid contents among different diet groups. The muscle protein, Val, Ile, His, essential amino acids and total amino acids significantly increased （P〈0.05） with the addition of 0.04% chlorogenic acid. Hydroxyproline and the collagen content of muscle increased （P〈0.05） with the addition of 0.02% chlorogenic acid. In conclusion, the results above showed that the growth, non-specific immunity ability and the meat quality of juvenile grass carp could be improved with the addition of chlorogenic acid in diets. Therefore, the recommended level of chlorogenic acid in juvenile grass carp diet should be 0.04%.