邻苯二甲酸二乙基己酯对鲤非特异性免疫的影响及遗传毒性

EFFECT OF DEHP ON NON-SPECIFIC IMMUNE FUNCTION AND GENETIC TOXICITY OF CYPRINUS CARPIO LINNAEUS

以水和吐温–80为对照,用5、20、80、160 mg/L的邻苯二甲酸二乙基己酯(DEHP)对鲤染毒20d,研究了DEHP对鲤非特异性免疫功能的影响及遗传毒性。结果表明:吐温–80组与水对照组相比除红细胞总核异常率显著升高外,所测定的各项指标差异均不显著。80、160 mg/L组白细胞吞噬活力、吞噬指数,血清抗菌活力,溶菌酶活性均显著低于水对照组和吐温–80组(P<0.05或P<0.01),且20 mg/L组白细胞吞噬活力、吞噬指数显著低于水对照组。与吐温–80组相比,160 mg/L组血清C3含量显著降低,5、20、80 mg/L组C3含量,5、20、80、160 mg/L组C4含量则无显著性差异。20、80、160 mg/L组C3含量,160 mg/L组C4含量显著低于水对照组(P<0.05或P<0.01)。160 mg/L组红细胞微核率显著高于吐温–80组,80、160 mg/L组红细胞微核率显著高于水对照组。在DEHP实验浓度范围内,红细胞核异常率、总核异常率,肝细胞DPC系数均显著高于水对照组和吐温–80组。一定浓度的DEHP对鲤具有免疫毒性和遗传毒性。

To study the effect of DEHP [Di-（2-ethylhexyl） phthalate] on the nonspecific immune function and genetic toxicity in Cyprinus carpio, four experimental concentrations of 5, 20, 80 and 160 mg/L of DEHP were treated to the C. Carpio for 20 days, H2O and Tween-80 were used as the controls respectively. The results showed that, when compared with the two controls, all indexes showed no significantly difference except the total nuclear abnormal rate in erythro-cytes which significantly increased in the Tween-80 group. Phagocytic activity and index （PI） of leucocytes, antibacte-rial activity and lysozyme activity of serum in the 80, 160 mg/L of DEHP groups were significantly lower than those in the two controls, and also phagocytic activity and index （PI） of leucocytes in 20 mg/L group decreased remarkably （P〈0.05 or P〈0.01） when compared with the water control group. When compared with the tween-80 group, the C3 content in serum of the 160 mg/L group decreased significantly. However, the C3 content in the groups of 5, 20 and 80 mg/L, and the C4 content in the groups of 5, 20, 80 and 160 mg/L showed no significantly differences. When compared with the water control group, the C3 content in the groups of 20, 80 and 160 mg/L, and the C4 content in the group of 160 mg/L decreased remarkably （P〈0.05 or P〈0.01）. Micronuclei rate of erythrocytes in the group of 160 mg/L was significantly higher than that in the group of tween-80, and micronuclei rate of erythrocytes in the group of 80 and 160 mg/L was significantly higher than that in the water control group. In the range of the DEHP concentration, the rate of nuclear abnormalities and total nuclear abnormalities of erythrocytes and the DPC coefficient of hepatocyte all increased significantly when compared with the two control groups. The immune and genetic toxicity on carp can be caused by a certain concentration of DEHP.