血浆微RNA-224在结直肠癌中的诊断价值

Diagnostic value of plasma microRNA-224 in colorectal cancer

目的 :探讨血浆中微RNA-224(microRNA-224,miR-224)对结直肠癌的诊断价值。方法 :留取12例结直肠癌患者的癌组织及其配对的癌旁正常黏膜组织、50例结直肠癌患者的术前血浆及其中40例术后第7天血浆,并收集33例健康志愿者血浆作为对照。应用实时荧光定量PCR法检测以上标本中miR-224的表达水平,并分析其与临床病理特征的关系,评估miR-224对结直肠癌的诊断价值。结果 :miR-224在结直肠癌组织中的表达水平较正常黏膜组织明显上调(P=0.027 4)。结直肠癌患者术前血浆中miR-224的表达水平高于健康人群(P<0.05),其中40例结直肠癌患者术后血浆中miR-224的表达水平较术前明显降低(P=0.005 3),而术前血浆中miR-224的表达水平与患者淋巴结转移及Dukes分期有关(P<0.05)。miR-224的受试者工作特征(receiver operating characteristic,ROC)曲线下面积为0.794,区分结直肠癌患者和健康人群的敏感度和特异度分别为60.0%和85.1%。结果 :血浆miR-224作为新的生物学指标,可能对于结直肠癌的诊断具有一定价值。

Objective： To explore the diagnostic value of plasma microRNA-224 （miR-224） in colorectal cancer. Methods： The tumor tissues and the adjacent normal mucosa tissues from 12 patients with colorectal cancer, the preoperative plasma specimens from 50 patients with colorectal cancer and their postoperative （at day 7 after operation） plasma specimens （only 40 cases among them） were collected. At the same time, the plasma specimens from 33 healthy volunteers were used as the controls. The expression of miR-224 in these samples was measured by real-time fluorescence quantitative-PCR, and its relationship with the clinical characteristics of patients with colorectal cancer was analyzed. Results： The expression of miR-224 in colorectal cancer tissues was up-regulated as compared with that in normal mucosa （P = 0.027 4）. The expression level of miR-224 in preoperative plasma from the patients with colorectal cancer was higher than that from healthy volunteers （P 〈 0.05）. As compared with the preoperative plasma, the expression of miR-224 in postoperative plasma from 40 cases was decreased （P = 0.005 3）. The expression of miR-224 in preoperative plasma had a signi？cant impact on lymphatic metastasis and Duke’s stage （P 〈 0.05）. For discriminating colorectal cancer patients from healthy volunteers, the area under the receiver operating characteristic curve was 0.794, and the sensitivity and specificity were 60.0% and 85.1%, respectively. Conclusion： Plasma miR-224, as a new biomarker, may be of diagnostic value in colorectal cancer.