髓样分化因子88对姜黄素诱导肝星状细胞凋亡的影响

Effect of myeloid differentiation factor 88 on the hepatic stellate cells apoptosis induced by curcumin

目的观察髓样分化因子88在姜黄素促进肝星状细胞(HSC)凋亡中的作用。方法体外培养大鼠肝星状细胞株HSCT6,并分为空白对照组、Control siRNA组、MyD88 siRNA干扰组、姜黄素组、姜黄素+Control siRNA组、姜黄素+MyD88 siRNA干扰组,siRNA处理组给予siRNA干扰48 h后,姜黄素组加入姜黄素作用24 h,各组均在收集细胞前12 h给予LPS诱导,收集各组细胞,Western blotting法检测MyD88蛋白表达;流式细胞术检测细胞凋亡。结果 MyD88 siRNA干扰、姜黄素均可降低MyD88蛋白的表达(P<0.05),同时给予MyD88 siRNA干扰和姜黄素作用时与单独给予姜黄素比较,MyD88蛋白下降更明显(P<0.05)。MyD88siRNA干扰后HSCs凋亡率无明显增加(P>0.05),给予姜黄素处理HSCs凋亡率增加(P<0.05),且姜黄素+MyD88 siRNA干扰组的凋亡率升高更明显。结论降低MyD88表达可加强姜黄素促进HSCs凋亡的作用。

Objective To observe the effects of MyD88 on hepatic stellate cells （HSCs） apoptosis induced by curcumin and to investigate its mechanism.Methods HSCs were divided into control group,control siRNA group,MyD88 siRNA group,curcumin group,curcumin ＋ control siRNA group,curcumin ＋ MyD88 siRNA group.SiRNA groups were interfered by siRNA for 48 hours,curcumin was added into curcumin groups for 24 hours to collect cells.LPS were put into all groups,then incubated HSCs with LPS for 12 hours.The expression of MyD88 protein was observed by Western blotting,cell apoptosis was detected by flow cytometry.Results Both MyD88 siRNA and curcumin could down-regulate the expression of MyD88 protein （P ＜ 0.05）,the decrease of MyD88 protein was more obviously observed in MyD88 siRNA ＋ curcumin group.The apoptosis rate of HSCs in MyD88 siRNA ＋ curcumin group was higher than that in curcumin group （P ＜ 0.05）,while the rate was hardly raised in MyD88 siRNA group.Conclusion Decrease of MyD88 could increase the apoptosis of HSCs induced by curcumin.