血管紧张素Ⅱ对大鼠肾小球内皮细胞单核细胞趋化蛋白1表达的影响

The Effect of Angiotension Ⅱ on the Expression of MCP-1 in Rat Glomerular Endothelial Cells

目的观察血管紧张素Ⅱ(AngⅡ)对大鼠肾小球内皮细胞(GEC)单核细胞趋化蛋白1(MCP-1)表达的影响,探讨其相关作用机制。方法对大鼠GEC进行分离培养与鉴定;采用Western blot法检测大鼠GEC炎性因子MCP-1蛋白的表达;RT-PCR和Western blot法检测血管紧张素Ⅱ1型受体(AT1R)的mRNA和蛋白水平。结果与正常对照组比较,施加AngⅡ刺激因素后MCP-1表达量明显增高,呈剂量(10-7mol/L^10-5mol/L)依赖效应;10-5mol/L AngⅡ作用下,大鼠GEC的AT1R mRNA和蛋白水平明显增加,成时间依赖效应;10-6mol/L AT1R拮抗剂替米沙坦(TEL)可以抑制AngⅡ(10-5mol/L)的作用,MCP-1的表达量下降。结论 AngⅡ通过上调大鼠GEC的AT1R水平,使大鼠GEC的MCP-1表达量增加。

Aim To investigate the effect of angiotension Ⅱ（ AngⅡ） on the expression of monocyte chemoattractant protein-1（ MCP-1） in rat glomerular endothelial cells（ GEC） and the related mechanism. Methods Primary GEC were isolated from Sprague-Dawley（ SD） rats. The synthesis of MCP-1 in rat GEC was determined by Western blot method. RT-PCR method was used to detect the expression of angiotensin Ⅱ type 1 receptor（ AT1R） mRNA. Results AngⅡ stimulation increased the synthesis of MCP-1 and promoted the expression of AT1 R mRNA in rat GEC.Telmisartan（ TEL）,an AT1 R blocker,blocked the effect of AngⅡ on rat GEC and decreased the expression of MCP-1.Conclusion AngⅡ induces MCP-1 production in rat GEC mediated by the AT1 R.