动脉粥样硬化大鼠Chemerin与炎症的相关性

Relationship Between Chemerin and Inflammation in Rats with Atherosclerosis

目的探讨动脉粥样硬化大鼠血浆Chemerin及主动脉Chemerin基因和Chemerin受体基因表达与炎症的相关性。方法健康雄性Wistar大鼠82只,分为对照组、辛伐他汀治疗组及高脂组,喂养14周处死,行心脏采血、分离胸主动脉及腹主动脉备HE染色及RT-PCR,ELISA法检测三组大鼠血浆Chemerin、hs-CRP、IL-6、TNF-β水平,半定量RT-PCR技术检测三组大鼠主动脉Chemerin及Chemerin受体mRNA水平。结果高脂组大鼠动脉粥样硬化形成良好。三组大鼠间血浆Chemerin、hs-CRP、IL-6、TNF-β水平差异具有统计学意义,呈高脂组>辛伐他汀治疗组>对照组(P<0.05);对照组、高脂组及辛伐他汀治疗组大鼠血浆Chemerin均与hs-CRP呈正相关,r值分别为0.664、0.804和0.709(P<0.05);三组大鼠间主动脉Chemerin mRNA及Chemerin受体mRNA表达水平差异具有统计学意义,呈高脂组>辛伐他汀治疗组>对照组(P<0.05);高脂组、辛伐他汀治疗组Chemerin mRNA及Chemerin受体mRNA表达水平与hs-CRP、IL-6、TNF-β呈正相关(P<0.05)。结论动脉粥样硬化大鼠血浆Chemerin水平、主动脉Chemerin及Chemerin受体mRNA表达水平与炎症密切相关,这提示Chemerin-Chemerin受体通路在联系炎症与动脉粥样硬化之间发挥重要的作用。

Aim To explore the relationship of plasma chemerin level and the expression of chemerin and chemerin receptor mRNA with inflammation in rats with atherosclerosis. Methods 82 male Wistar rats were randomly divided into 3 groups： control group,high-fat group and simvastatin treatment group. The rats were fed for fourteen weeks. Cardiac blood samples and aorta samples were taken from the rats. The thoracic aorta and abdominal aorta were prepared for HE staining and RT-PCR. The plasma levels of chemerin,hs-CRP,IL-6 and TNF-β were measured by ELISA. Chemerin and chemerin receptor mRNA expression levels were determined by RT-PCR. Results There were significant differences among three rat groups on the plasma levels of chemerin,hs-CRP,IL-6 and TNF-β,which showed high-fat group simvastatin treatment group control group（ P〈0. 05）; Plasma chemerin levels of control group,high-fat group and simvastatin treatment group were positively correlated with hs-CRP（ r = 0. 664,0. 804 and 0. 709,P〈0. 05）. There were significant difference among three rat groups on chemerin and chemerin receptor mRNA expression levels,which showed high-fat group simvastatin treatment group control group（ P〈0. 05）. Chemerin and chemerin receptor mRNA expression levels of high-fat group and simvastatin treatment group were positively correlated with hs-CRP,IL-6 and TNF-β（ P〈0. 05）. Conclusion The plasma chemerin level and expression of the chemerin and chemerin receptor mRNA in rats with atherosclerosis were significantly correlated with inflammation. It implies that the pathway of chemerin-chemerin receptor may play pivotal roles in the pathogenesis and development of atherosclerosis through systemic inflammation.