摘要
目的通过比较不同临床类型冠心病患者外周血单核细胞亚型2（Mon2）的比例变化特征,揭示Mon2型单核细胞与冠心病临床表型的内在联系。方法利用流式细胞技术,以CD14＋CD163＋作为Mon2单核细胞的标记,检测90例冠心病患者[病例组,稳定型心绞痛（SAP）组28例;不稳定型心绞痛（UAP）组30例;急性心肌梗死（AMI）组32例]与5例健康对照个体（对照组）外周血Mon2单核细胞的比例以及CD163平均荧光强度（MFI）分布差异;回归分析Mon2单核细胞亚型比例、CD163荧光强度与冠状动脉狭窄程度的相关性。结果 Mon2型单核细胞比例、CD163荧光强度在病例组与对照组分布差异有显著性（P〈0.001）,且Mon2型单核细胞比例在SAP组为24.3%（19.1%-29.5%）,UAP组为11.1%（6.1%-16.9%）,AMI组为8.2%（3.3%-13.1%）,差异具有高度显著性（P=0.001）。循环血单核细胞CD163的平均荧光强度在SAP组为1.58（1.38-1.78）,UAP组为1.21（1.04-1.39）,AMI组为1.10（0.94-1.27）,差异具有显著性（P=0.008）;Mon2型单核细胞比例及CD163荧光强度与冠状动脉评分无相关性。结论冠心病患者外周血Mon2分布比例与冠心病患者表型严重程度密切相关。
Aim The roles of monocyte/macrophage subsets（ Mon1,Mon2,Mon3） are different on atherosclerotic inflammation. The aim of this article is to identify the distribution of monocyte Mon2 in patients with different clinical types of coronary artery disease,and to reveal correlation between monocyte Mon2 and atherosclerosis. Methods Totally 90 patients with coronary artery disease（ stable angina pectoris,SAP,28; unstable angina pectoris,UAP,30; and acute myocardial infarction,AMI,32） and 5 controls were enrolled. Mon2 were evaluated with CD14＋CD163＋using flow cytometry. Correlation between Mon2 and coronary score was evaluated using unitary linear recursive analysis.Results Monocyte Mon2 proportions were significantly different between patients with coronary artery disease and controls（ P =0.001）,furthermore among patients with coronary artery disease,Mon2 proportions had significant difference in SAP（ 24.3 %,19.1% -29.5%）,UAP（ 11.1 %,6.1% -16.9%） and AMI group（ 8.2 %,3.3% -13.1%）,respectively. Value of CD163 MFI had significant difference in SAP（ 1. 58,1. 38 - 1. 78）,UAP（ 1. 21,1. 04 - 1. 39） and AMI group（ 1. 10,0. 94 - 1. 27）,respectively. However,no correlation was found between coronary score and Mon2 proportion and CD163 MFI. Conclusions The distribution of monocyte Mon2 varies was correlated with type of coronary artery disease.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2014年第8期813-816,共4页
Chinese Journal of Arteriosclerosis
基金
广东省自然科学基金(S2011010005099)
