Krüppel样因子5在小鼠舌黏膜癌变过程中的表达

Expression of KLF5 in 4NQO-induced mouse tongue carcinogenesis

目的检测Krüppel样因子5(Krüppel-like factor 5,KLF5)在小鼠舌黏膜癌变模型中的表达,探讨KLF5与口腔黏膜癌变的关系。方法将42只C57BL/6J小鼠随机分为阴性对照组、4-硝基喹啉-1-氧化物(4NQO)处理组。阴性对照组不做任何处理,4NQO组以4NQO水溶液(50μg/ml)代替饮用水饲养,共16周。实验第24周末处死全部动物,取舌组织进行HE染色及KLF5平均光密度值分析。结果 HE染色显示,对照组小鼠舌背上皮为正常角化的复层鳞状上皮,饮用4NQO水溶液的小鼠舌黏膜上皮出现了不同程度的异常增生及鳞癌。平均光密度测定显示,与正常口腔黏膜上皮相比,KLF5在重度异常增生和鳞癌中的表达明显下降(P<0.01)。结论 KLF5可能在口腔黏膜上皮癌变过程中作为肿瘤抑制基因发挥重要作用。

Objective To examine the expression of KLF5 in 4NQO-induced mouse tongue carcinogenesis. Methods Forty-two C57BL/6J mice were divided into two groups, control group （without any treatment） and 4NQO group, in which the mice were given 4NQO（50μg/ml） in drinking water for 16 weeks. At the end of 24thweek , the mice were sacrificed and their tongues were harvested for HE staining and immunohistoehemistry. Results HE staining showed that normal stratified squamous epithelium were observed in the tongues of the control, and mild to severe dysplasia and even to squamous cell carcinoma lesions appeared in the tongues of 4NQO group. Immunohistochemistry results showed that the KLF5 displayed a strong nuclear staining in normal tongue mucosa. The expression of KLF5 markedly decreased in the severe dysplasia to squamous cell carcinomas, which was significantly different with that in the control group （ P 〈 0.01 ）. Conclusion KLFS may serve as a tumor suppressor gene in oral carcinogenesis.