大鼠正畸牙移动过程中压力侧牙周组织NALP3的表达

NALP3 expression in periodontal tissues of pressure side during orthodontic tooth movement in rats

目的观察大鼠正畸牙移动过程中压力侧牙周组织NALP3的表达,探讨NALP3在正畸牙移动过程中的作用。方法将50只6周龄雄性SD大鼠随机分为10组,每组5只。以左上第一磨牙作为实验牙,分别加力0、1、3、6、12h、1、3、7、14、21d。制备不同时间牙周组织切片,进行HE染色和NALP3免疫组化染色。结果正畸牙移动过程中压力侧牙周组织NALP3的表达发生变化。加力后牙周膜NALP3表达即增加,3h达到小高峰,随后降低,1d后表达再次增加,7d后达到最高峰。结论 NALP3参与了正畸牙移动过程中牙周组织的改建。

Objective To examine the NALP3 expression in periodontal tissues of pressure side during orthodontic tooth movement in rats, and to explore the role of NALP3 during orthodontic tooth movement. Methods Fifty male SD rats aged 6 weeks old were randomly divided into 10 groups of 5. The upper left first molar was experimental tooth, which was pulled mesially by orthodontic force for 0, 1, 3, 6, 12 hours, 1, 3, 7, 14, 21 days respectively. Routine five-micrometer paraffin tissue sections were prepared and HE staining and immunohistochemical staining for NALP3 were carried out. Results The expression of NALP3 increased in pressure zone of periodontal ligament after loading, reaching a peak on the third hour and then decreased. The NALP3 expression increased again after one day, and reached the peak on the seventh day. Conclusion NALP3 was involved in the process of periodontal tissue remodeling during orthodontic tooth movement in rats.