组蛋白去乙酰化酶抑制剂对乙型肝炎病毒复制的影响

Effect of histone deacetylase inhibitor on HBV replication

目的:研究SIRT1抑制剂sirtinol、曲古抑菌素A(trichostatin A,TSA)对乙型肝炎病毒(hepatitis B virus,HBV)复制的影响,并探讨组蛋白去乙酰化酶(histone deacetylase,HDAC)在HBV复制过程中的作用。方法:运用MTS分析HepG2.2.15细胞对sirtinol、TSA的耐受程度;real-time PCR和Southern blot验证sirtinol和TSA对HepG2.2.15细胞中HBV复制中间体表达的影响;Western blot和ELISA分析sirtinol对HBV核心蛋白(HBc)和HBsAg、HBeAg表达的影响。结果:sirtinol能抑制HBV复制中间体、HBc的表达和HBsAg、HBeAg的分泌,TSA可以促进HBV的复制。结论:SIRT1抑制剂sirtinol具有抗病毒作用。

Objective：To investigate the effect of SIRTI inhibitor sirtinol,trichostatin A（TSA） on hepatitis B virus（HBV） replication and to discuss the role of histone deacetylase in the process of HBV replication. Methods ：The tolerance degree of HepG2.2.15 cell to sirtinol and TSA was determined by MTS assay. The effects of sirtinol and TSA on the expression of HBV replicative intermediates were measured by real-time PCR and Southern blot. The effects of sirtinol and TSA on the expression of HBc, HBsAg and HBeAg were analyzed by Western blot and ELISA. Results：Sirtinol inhibited the expression of HBV replicative intermediates and HBc as well as the secretion of HBsAg and HBeAg. TSA promoted the HBV replication. Conclusion：SIRT1 inhibitor sirtinol may have anti-HBV potentiality.