心肌梗死模型大鼠心功能与血管活性物质释放关键蛋白NSF-siRNA

Cardiac function of myocardial infarction rat models and NSF-siRNA, a key protein released from vesoactive substance

背景:如何通过有效的干预手段,降低心血管疾病发病率与死亡率,已经成为一个日益迫切的重大公共卫生问题。目的:探索腺病毒介导的血管活性物质释放关键蛋白NSF-siRNA对心肌梗死模型大鼠心脏功能的影响。方法:36只成年SD大鼠,经结扎左冠状动脉前降支建立急性心肌梗死模型。心电图确定模型成功后,分别给予大鼠心脏左室壁梗死区周围局部注射NSF-siRNA腺病毒(实验组)、阴性腺病毒(对照组)及生理盐水(生理盐水组)。2周后通过无创超声心动图测定心脏左室射血分数(LVEF)值;并通过右颈外动脉置管,连接BL-420生物机能实验系统测定左心室舒张末期压(LVEDP)及心室内压力变化率峰值(dp/dtmax)评价心功能;随后取材、连续切片,观察心肌梗死范围。结果与结论:2周时实验组左室射血分数值与对照组及生理盐水组相比明显增加(P<0.05);实验组左室舒张末压与对照组及生理盐水组相比显著降低(P<0.05);实验组左室最大压力上升速率与对照组及生理盐水组比较明显增加(P<0.05);心肌梗死面积各组比较差异均无显著性意义(P>0.05)。结果可见梗死局部周围注射腺病毒介导的NSF-siRNA表达载体,能显著改善模型大鼠心肌梗死后2周左室射血分数、左心室舒张末期压、心室内压力变化率峰值3项心功能指标,但对心肌梗死面积无明显影响。

BACKGROUND：How to reduce the incidence and mortality of cardiovascular diseases is an urgent concern in the field of public health. OBJECTIVE：To explore the influence of adenovirus-mediated NSF-siRNA release from vesoactive substance on the cardiac function of a rat model of myocardial infarction. METHODS：A total of 36 adult Sprague-Dawley rats were applied to establish acute myocardial infarction models by ligating the anterior descending branch of the left coronary artery. After the model was determined by electrocardiogram successful y, NSF-siRNA adenovirus （experimental group）, negative adenovirus （control group） and normal saline （normal saline group） were injected near the infarct area of the left ventricle of rats respectively. After 2 weeks, the left ventricular ejection fraction （LVEF） was tested with noninvasive ultrasonic cardiogram. Meanwhile, the left ventricular end-diastolic pressure （LVEDP） and maximum pressure rising speed of left ventricular （dp/dt max） were detected by connecting the right external carotid artery place pipe to the BL-420 biological function experiment system, to evaluate the cardiac function. Subsequently, the rat heart was harvested for serial sections to observe the infarcts range.