体表脉冲电刺激对昆明小鼠心电活动的影响

Effect of surface electric-impulse stimulation on cardiac electrical activity of Kunming mice

背景:使用不同强度、频率及时间的电刺激作用于人体,可能产生各种不同的病理生理反应。目的:观察体表脉冲电刺激对小鼠心律及心率的影响。方法:将30只昆明小鼠随机分为3组,每组10只,用BL-420F型生物机能实验系统提供刺激电源,对3组昆明小鼠分别予以不同电压、不同时间、不同频率的体表电刺激,然后观察Ⅱ导联心电图、小鼠得全身反应与局部变化。结果与结论:≤10 V的电刺激对小鼠心率无明显影响,并且未出现心律失常;电压≥15 V时,电刺激可使小鼠心率减慢,停止30 min后心率恢复正常;电压≥35 V时,小鼠出现心率减慢和心律失常,并且停止刺激后心率不能恢复正常;电压>75 V后,小鼠出现室颤。电压为10 V时,脉冲电刺激时间和频率变化对小鼠心电活动影响不显著。说明脉冲电刺激对小鼠心电活动的影响主要由所用电压高低不同引起,电压≤10 V时对小鼠心电活动无影响;脉冲电刺激时间与电场频率对小鼠心电活动影响不明显。

BACKGROUND： Electrical stimulation at different intensity, frequency and time on the human body may produce a variety of pathophysiological reactions. OBJECTIVE： To observe the effects of surface electric-impulse stimulation on heart rhythm and heart rate in mice. METHODS： Thirty Kunming mice were randomly divided into three groups, each group contained 10 mice. Electrical stimulation at different voltage, time and frequency was respectively applied to the three groups. The stimulus power was supplied by BL-420F Data Acquisition ＆ Analysis System. The II lead electrocardiogram was recorded. The systemic reactions and local body changes of mice were observed. RESULTS AND CONCLUSION： Electrical stimulation at the voltage less than or equal to 10 V had no effect on heart rate, and there was no arrhythmia. When the voltage was greater than or equal to 15 V, electrical stimulation slowed down heart rate, which was restored to normal 30 minutes later. When the voltage was greater than or equal to 35 V, arrhythmia and slow heart rate occurred in mice, and the heart rate can not get back to normal. Also, some mice got ventricular fibrillation after electrical stimulation at the voltage greater than 75 V. However, when voltage was equal to 10 V, the effect of electrical stimulation at different frequency or time on cardiac electrical activity was not significant. The effect of electric-impulse stimulation by body surface on heart rhythm and heart rate in mice mainly depends on the discretion of electrical stimulation voltage. Less than or equal to 10 V, electric-impulse stimulation has no effect on heart rhythm and heart rate; the frequency or time of electric-impulse stimulation almost has no significantly effect on heart rhythm and heart rate in mice.