颈椎不稳与椎动脉缺血动物模型的实验与构建

Establishment of animal models of cervical instability and vertebral artery ischemia

背景:颈椎病是颈椎椎间盘组织退行性改变及其继发病理改变累及其周围组织结构。颈椎不稳与椎-基底动脉缺血的动物模型是研究颈椎病病理生理以及治疗的关键。目的:对颈椎不稳与椎-基底动脉缺血的动物模型进行阐述,探讨动物模型实验研究的新进展。方法:由作者应用计算机检索PubMed数据库及CNKI数据库1979至2012年,在英文标题和摘要中以"cervical instability,basal-vertebral artery ischemia"和"animal model"检索,中文文献检索以"颈椎不稳,椎动脉缺血,动物模型"为关键词,选择内容与颈椎不稳、椎动脉缺血、动物模型相关的文章,同一领域文献则选择近期发表或发表在权威杂志文章,共纳入43篇文献。结果与结论:目前建立合适的理想的颈椎病模型尚需继续探讨。利用动物颈椎病模型进行的研究,目前多集中在病因、发病机制和生物化学等方面,由于颈椎病的病因及发病机制目前尚不清楚,现有的造模方法难以全面重现人类颈椎病模型,故模型成立、阳性率及造模时间合适的、理想的颈椎病模型尚需探讨。

BACKGROUND：Cervical spondylosis refers to cervical intervertebral spondylotic myelopathy and secondary degenerative changes, as wel as pathological changes in surrounding tissue structures. Establishing animal model of cervical instability and vertebral-basilar artery ischemia is the key in the studies addressing cervical spondylosis pathophysiology and treatment. OBJECTIVE：To establish animal model of unstable cervical spine and vertebral-basilar artery ischemia, and explore new progress of animal model imitation study. METHODS：A computer-based retrieval of PubMed database and CNKI database was performed for articles published from 1979 to 2012. The key words were“cervical instability, basal-vertebral artery ischemia, animal model”in English and Chinese. The articles about cervical instability, basal-vertebral artery ischemia, and animal model were screened, and those published recently or in authorized journals were preferred in the same field. Final y 43 articles were included in this study. RESULTS AND CONCLUSION：An ideal animal model of cervical disease is needed. Animal model of cervical diseases is often used for the study of disease causes, onset mechanism and biochemistry. As the causes and mechanism of cervical diseases remain unclear, the existing modeling method cannot duplicate human cervical diseases, so further studies are needed to explore the establishment of models, positive rate and modeling time.