多光子显微镜诊断早期消化系统肿瘤的研究进展

Application of multiphoton microscopy diagnosis of early phase tumors of digestive system

近年来消化系统肿瘤的发病率和死亡率越来越高,而实现肿瘤的早期诊断可提高患者手术切除率进而延长患者的生存时间。多光子显微镜(multiphoton microscopy,MPM)与传统的诊断方法相比,对组织的穿透深度上得到了明显的提高,对组织的光毒性和光漂白作用减少。它与二次谐波显微镜相结合,可观察到组织中的胶原纤维的变化。MPM在细胞水平实现肿瘤的早期诊断。因此MPM以其独有的高分辨、实时、无损、深层的光谱分辨成像特点,在消化系统肿瘤的早期诊断中是极具潜力的。文中根据国内外的研究成果,针对MPM诊断早期消化道肿瘤的研究进展作一综述。

The morbidity and mortality of tumors of digestive system is much higher in recent years .Realizing diagnosis of early phase tumors can improve the surgical resection rate and prolong survival time of patients .Compared with traditional diagnosis methods, multiphoton microscopy has got an obvious improvement in the penetration depth .Furthermore, phototoxicity and photo-bleaching has been largely reduced .Combining with the second harmonic microscopy , the change of collagen fibers can be observed in the organization.MPM can achieve early diagnosis of cancer at the cellular level .Therefore, multiphoton microscopy , with the imaging characteristics of unique high resolution , real-time, nondestructive , deeply spectral resolution , is of great potential in the diagnosis of early stage tumors of digestive system .Based on the latest research results , this review summarizes the latest progress of multiphoton microscopy diagnosing early phase of gastrointestinal tumor .