CD9蛋白在胰腺癌组织中的表达及其临床意义

Expression and its clinical significance of CD9 protein in pancreatic cancer

目的 分析CD9蛋白与胰腺癌进展及预后的关系.方法 收集2005年9月至2009年12月间90例胰腺癌患者手术切除的癌组织和癌旁组织标本及临床资料,采用免疫组织化学方法,检测胰腺癌组织和癌旁组织标本中CD9蛋白的表达情况,分析其与胰腺癌患者临床病理特征关系,组间率的比较行卡方检验.对90例患者的术后生存时间进行生存分析.结果 胰腺癌组织中CD9蛋白的高表达率（64.4％,58/90）显著高于癌旁组织（45.6％,41/90）,差异有统计学意义（χ2=6.847,P＜0.05）.分化程度高、无淋巴结转移的胰腺癌组织中CD9蛋白多呈高表达[74.6％（50/67）比39.1％（9/23）,χ2=9.554,P＜0.01;50.0％（17/34）比73.2％（41/56）,χ2=5.856,P＜0.05],CD9表达与性别、年龄无相关性（P均＞0.05）;高表达CD9的患者较低表达者的总生存时间和无进展生存时间显著延长（中位总生存时间为33.0个月比7.0个月,χ2=15.400,P＜0.01;中位无进展生存时间为30.5个月比5.0个月,χ2=13.750,P＜0.01）.结论 CD9蛋白是一种影响胰腺癌侵袭能力的相关蛋白,在胰腺癌发展和转移中起着一定的作用,一定程度上能帮助判断预后情况.

Objective To examine the expression of CD9 protein in pancreatic cancer tissues and adjacent tissues,and to analyze its relation with the progress and prognosis of pancreatic cancer.Methods From September 2005 to December 2009,surgical resected cancer tissues and adjacent tissues of 90 patients with pancreatic cancer and their clinical data were collected.The expression of CD9 protein in pancreatic cancer tissues and adjacent tissues was detected by immunohistochemistry,and its relationship with clinicopathological features was analyzed.Chi-square test was performed for comparison of ratios between groups.Overall survival （OS） analysis of 90 patients after surgery was performed.Results The high expression rate of CD9 protein （64.4%,58/90） in cancer tissue was significantly higher than that in cancer adjacent tissue （45.6%,41/90）,the difference has statistically significant （χ2 =6.847,P〈0.05）.CD9 protein was highly expressed in most of pancreatic cancer tissue which was well differentiated or without lymph node metastasis （74.6% （50/67） vs 39.1% （9/23）,χz =9.554,P〈0.01; 50.0%（17/34） vs 73.2%（41/56）,χ2 =5.856,P〈0.05 respectively）.However,the expression of CD9 was not correlated with gender and age （both P〉0.05）.OS and progression-free survival （PFS） of the patients with CD9 highly expressed were significantly longer than those with low expression of CD9 （median OS：33.0 months vs 7.0 months,χ2 =15.400 P〈0.01.Median PFS：30.5 months vs 5.0 months,χ2 =13.750,P〈0.01）.Conclusion CD9 protein is a kind of protein related with the invasive ability of pancreatic cancer,which may play a role in progression and metastasis of pancreatic cancer and can help to determine the prognosis to a certain extent.