GIRK4在肥胖大鼠模型心肺组织中的表达研究

Study of GIRK4 Expression in Heart and Lung Tissue of Diet- Induced Obesity Rats

目的研究饮食诱导肥胖大鼠G-蛋白偶联(门控)内向整流钾离子通(GIRK4)基因在心肺组织中的表达,探讨GIRK4与肥胖的相关性。方法 30只SD大鼠,雌雄各半,10对照组(n=10),予以普通饲料饲喂作为,实验组(n=20),以高脂肪高热量饮食饲喂8周,建立肥胖大鼠模型,肥胖大鼠体重超过正常饮食饲喂大鼠20%以上为成功模型,处死大鼠,提取两组大鼠的心肺组织,分别提取心肺的总蛋白,应用Western blot检测技术,检测GIRK4蛋白表达,Quantity One软件处理实验结果。结果①15只大鼠进入肥胖组(体重大于正常对照组20%);②肥胖组血清总胆固醇及甘油三酯水平高于对照组(P<0.05),高脂高糖饮饲喂建立大鼠模型,肥胖大鼠心、肺中表达GIRK4蛋白较正常饲喂大鼠低,两组间差异无统计学意义(P>0.05)。结论GIRK4在大鼠心、肺均有表达,可能与肥胖时心律失常及肺损伤相关。

Objective To investigate GIRK4（G protein-coupled inward rectifier K ＋ channels 4 gene） expression in heart and lung tissue of diet induced obesity rat model,and speculate the correlation between GIRK4 and obesity.Methods Thirty SD rats were randomly assigned to obesity group （n =20）as fed by high-fat and high-sugar diet for establishment of obesity model and normal control group （n =10）as fed by normal diet.The rats were sacrificed followed by separation of heart and lung tissue,Protein of heart and lung tissue was extracted for detection of GIRK4 expression via Western blot.The weight,length and fat coefficient were compared.Result：Obese rats yielded markedly higher weight（381.78 ± 73.58g vs 285.57 ± 59.17g）,length （24.87 ± 2.23cm vs 22.40 ± 2.01cm）,fat coefficient （3.17 ± 1.06 vs 1.10 ± 0.43） （P ＜ 0.05,respectively）.Not significantly statistic difference of GIRK4 expression in heart and lung tissue between obesity group and normal control group （P ＞ 0.05） was found.Conclusion The GIRK4 expression in heart and lung tissue may be associated with arrhythmia and pulmonary injury in obesities and obesity induced by diet.