溶瘤腺病毒介导IL-24增强黑素瘤细胞MV3放疗敏感性的研究

Sensitivity to the melanoma cell line MV3 radiotherapy enhanced by oncolytic adenovirus mediated interleukin-24

目的:研究溶瘤腺病毒介导IL-24(ZD55-IL-24)对黑素瘤细胞MV3放疗敏感性的影响。方法:将对数生长的MV3细胞分为ZD55-IL-24联合放疗组、ZD55-IL-24组、放疗组、PBS对照组。应用免疫细胞化学法检测黑素瘤MV3细胞中Bax和Bcl-2凋亡相关蛋白的表达:应用Western blot法检测E1A蛋白及Bax、Bcl-2、Caspase-3凋亡相关蛋白的表达。结果:免疫细胞化学法检测联合治疗组Bax染色强度最大,Bcl-2染色强度最小。ZD55-IL-24联合放疗作用的MV3细胞高效表达E1A,较其它组Bax的表达量增加,Bcl-2的表达量降低,Caspase-3活化更明显。结论:ZD55-IL-24联合放疗有明显的协同杀伤黑素瘤细胞的作用。

To investigate the sensitivity to the melanoma cell line MV3 radiotherapy enhanced by oncolytic adenovirus mediated interleukin-24 （ZD55-IL-24）. Methods： The logarithmic phase cells of melanoma cell line MV3 were divided into ZD55-IL-24 ＋ radiotherapy group, ZD55-IL-24 group, radiotherapy group and PBS control group. Bax and Bcl-2 apoptosis related proteins of MV3 cells were measured by immunocytochemical staining. The expression of E1A proteins,Bax, Bcl-2 and Caspase-3 apoptosis related proteins were detected by Western blotting analysis. Results： The staining intensity of Bax was the strongest and the staining of Bcl-2 was weakest in ZD55-IL-24 plus radiotherapy group, when measured by immunocytochemical staining. Western blotting analysis showed that the expression of EIA was highest and the expression of Bax was also increased in ZD55-IL-24 plus radiotherapy group than other groups. The expression of Bcl-2 was decreased and the activation of Caspase-3 was more significant. Conclusion： ZD55-IL-24 conjugated with radiotherapy has a synergic effect in the killing of melanoma cells.