精子相关抗原9在宫颈腺癌中的表达及其临床意义

Expression and significance of sperm associated antigen 9in the tissue of cervical adenocarcinoma

目的研究宫颈腺癌组织中精子相关抗原9（SPAG9）的表达及其临床应用价值。方法采用免疫组化链霉亲和素-生物素-过氧化物酶复合物技术（streptavidin biotin-peroxidase complex method,SABC）检测50例宫颈腺癌和18例正常宫颈组织中SPAG9蛋白的表达。结果 SPAG9在宫颈腺癌中的阳性表达率为100%,在正常宫颈组织中表达率为0。根据H-score值,不同病理分级宫颈腺癌中SPAG9表达比较,差异有统计学意义（t=0.661,P〈0.05）,但不同临床分期和盆腔淋巴结转移SPAG9表达比较,差异无统计学意义（t=0.705和1.874,P均〉0.05）。低分化宫颈腺癌患者SPAG9蛋白的表达[（3.25±0.47）分]与高、中分化[（3.18±0.36）分]比较,差异有统计学意义（P〈0.05）。有盆腔淋巴结转患者SPAG9蛋白的表达[（3.39±0.46）分]与无淋巴结转移[（3.18±0.29）分]比较,差异无统计学意义（P〉0.05）;Ⅱ~Ⅳ期宫颈腺癌患者SPAG9蛋白的表达[（3.43±0.42）分]与Ⅰ期[（3.07±0.35）分]比较,差异无统计学意义（P〉0.05）。结论 SPAG9蛋白在宫颈腺癌组织中存在高表达,可能在宫颈腺癌的发生和发展中起重要作用,具有成为其协助诊断和判断预后的分子标志物和靶向治疗靶点的潜能。

Objective To investigate the expression of sperm associated antigen 9 （SPAGg） in the cervical adenocarcinoma （CA） tissue and to disccuss the potential application value of SPAG9 for clinical work. Methods The method of immunohistochemistry staining was applied to determine the expression of SPAG9 in the tissue obtained from 50 patients with cervical adenocarcinoma and 18 patients without any cervical lesions. The immunohisto- chemistry H - score method was used to analyze the outcomes of the immunohistochemistry staining. Results Immunohistochemical results show that the rate of expressions of SPAG9 in the tissue of CA was 100% （50/50） , however, it was 0 （0/18） in the normal tissue. Results analyses revealed that SPAG9 expression was significantly associated with tumor grades. Conclusions The extent of the expression of SPAG9 in the cervical ad tissue indicates that it may play an significant role on the genesis and progression of CA. SPAG9 has the potential of becoming a biological marker for diagnosis and prognosis for cervical adenocarcinoma.