Caveolin-1与胰腺癌上皮间质转化及转移关系的研究

The relationship between expression of caveolin-1 and epithelial-mesenchymal transition and metastasis in pancreatic cancer

目的 研究细胞窖蛋白-1（caveolin-1,Cav-1）在胰腺癌上皮间质转化（epithelialmesenchymal transition,EMT）和转移中的作用及其相关机制.方法 免疫荧光技术、Western blot检测Cav-1及EMT分子标志物E-cadherin,β-catenin,vimentin,N-cadherin在高转移胰腺癌细胞系（L3.7,Panc02-H7）及低转移胰腺癌细胞系（COLO357,Panc02）中的表达,倒置相差显微镜观察上述胰腺癌细胞形态.Cav-1及相应的空载体质粒转染COLO357细胞,检测Cav-1及EMT分子标志物的表达,并观察细胞形态变化.结果 高转移胰腺癌细胞（L3.7,Panc02-H7）Cav-1及间质细胞标志物高表达,上皮细胞标志物低表达,而低转移胰腺癌细胞（COLO357,Panc02）Cav-1及上皮细胞标志物高表达,间质细胞标志物低表达.Cav-1质粒转染COLO357细胞后,Cav-1表达上调,E-cadherin表达降低而vimentin表达升高,且COLO357细胞由上皮细胞形态向间质细胞形态转变.结论 胰腺癌细胞中Cav-1表达升高与胰腺癌EMT及转移密切相关.

Objective To study the relationship between the expression of Caveolin-1 （Cav-1） and epithelial-mesenchymal transition（EMT） or metastasis in human pancreatic cancer.Methods The Cav-1 protein and EMT markers in highly metastatic pancreatic cancer cell lines （L3.7,Panc02-H7） and poorly metastatic pancreatic cancer cell lines （COLO357,Panc02） was detected by Western blotting and immunofluorescence.The morphology of the pancreatic cancer cells were observed under phase-contrast photomicrographs.The plasmids pcDNA3.1-caveolin-1 and control vector pcDNA3.1 were transfected into COLO357 cells by Lipofectamine LTX.The expression of Cav-1 protein,E-cadherin and vimentin were detected,and the morphology of COLO357 cells observed.Results L3.7 and Panc02-H7 cells had strong positive Cav-1 staining and high expression of mesenchymal marker （vimentin,N-cadherin） and low epithelial marker （E-cadherin,β-catenin）,whereas COLO357 and Panc02 cells with typical epithelial morphology were weak positive in Cav-1 staining and of low expression of vimentin,N-cadherin and high expression of E-cadherin,β-catenin.In Cav-1 transfected COLO357 cells vimentin expression increased,and E-cadherins decreased resulting in typical morphology changes of EMT.Conclusions Overexpression of caveolin-1 is correlated with epithelial-mesenchymal transition of pancreatic cancer cells and cancer metastasis.