枳壳甘草汤治疗脊髓综合征药效学分析研究

Pharmacodynamic analysis on optimization program of dosage ratio for Zhiqiao Gancao decoction

目的探讨"枳壳甘草汤"治疗脊髓综合征的剂量优化配比方案。方法取108只雄性SD大鼠,利用杠杆原理制备大鼠前脊髓综合征模型。随机分为8组(n=12),即模型组、阳性药(甲基强的松龙)组、中药组方(1、2、3、4、5、6)组;余12只为假手术组。中药组方各组以均匀设计的枳壳甘草汤的配比方灌胃,阳性药组以甲基强的松龙腹腔注射,模型及假手术组以同体积的新鲜蒸馏水灌胃,连续给药14天,每天1次。于造模后3 h,1、3、7、14 d的时间点分别采用BBB评分、CBS评分方法,对各组试验动物进行运动、神经功能评分。结果中药方枳壳甘草汤治疗大鼠前脊髓综合征临床效果满意,其中中药组4 BBB评分及CBS评分高于其余各组。结论中药组4(枳壳20 g,甘草5 g,当归25 g,莪术10 g,大黄30 g,芒硝15 g)为枳壳甘草汤的优化配比方案。

Objective To analyze optimization program of dosage ratio for Zhiqiao Gancao de-coction in treating spinal cord injury. Methods 108 male SD rats were prepared. The T13 spinal cord injury model in rats were induced by leverage theory. All the rats were randomly divided into eight groups （n=12）,model group,positive drug （methylprednisolone）group,TCM groups（1,2,3,4,5, 6）. The remaining 12 rats belonged to sham operation group. TCM groups were gavaged by uniform design Zhiqiao Gancao decoction. Positive group was injected intraperitoneally with methylpred-nisolone. Model and sham operation groups were given the same volume of fresh distilled water. The drugs were given continuously for 14 days,once a day. The BBB score and CBS score were used to evaluate the motor function and neurological function of the rats on 3 h,1 d,3 d,7 d,14 d after modeling. Results The efficacy of Zhiqiao Gancao decoction in treating spinal cord injury rats was satisfactory. BBB scores and CBS scores of TCM group 4 were higher than the other groups. Conclusion TCM group 4 （Zhiqiao 20 g,Gancao 5 g,Danggui 25 g,Ezhu 10 g,Dahuang 30 g,Mangxiao 15 g） is the optimized program of Zhiqiao Gancao decoction.