毕赤酵母发酵生产甲醇蛋白工艺条件的优化

Optimization of fermentation conditions for methanol protein production by Pichia pastoris

利用实验室选育驯化出的高产甲醇蛋白的毕赤酵母（Pichia pastorisYM 6C P02）作为甲醇蛋白生产菌种,对甲醇蛋白发酵生产过程中的关键因素进行较为全面的考察和优化,初步确定了摇瓶水平发酵各因素的最优条件为接种量8％,分段发酵温度采用0～36 h,30℃,36～64 h,28℃,初始pH值为5.0,初始甲醇添加量为1.2％,装液量为50 m L/250 m L摇瓶,转速为200r/m in.在此条件下,得到的甲醇蛋白最高产量（细胞干质量）为19.3 g/L.

PichiapastmisYM-SC P02 strain bred and dom esticated in laboratory with high methanolpmteh yield wasused form ethanolpmteh ptoduction.The key factom ofm ethanol pmtein ferm entation were investigated and optim ized,and the optim alfem entation conditions in the shake flask wem determ bed as follow s：hocuhm 8％,segmented ferm entation tem pemtum 0-36 h,30 ℃ and 36-34 h,28 ℃,hitialpH 5.0,hitialm ethanolad dition 1.2％,liquid vohm e 50 m 1/250 m lshake task,and mtate speed 200 r/m h.Under these conditiong the highestyield ofm ethanolpmteh （cell dry w eighO w as 19.3 g/L.