摘要
研究了5种天然色素-越橘红、红米红、黑加仑红、萝卜红、葡萄皮红的高效液相色谱检测法,样品处理后,甲醇-乙腈-甲酸为流动相进行梯度洗脱,在检测波长为515 nm条件下对5种目标物同时进行检测.试验表明,5种色素检测的相对标准偏差为5 7％～7 4％,回收率为74.1％～94.7％,检出限为5.6～7.5 mg/L.由此可知,本研究建立的天然色素检测方法简单快速,精确可靠,重复性好.
A simple and accurate method for the determination of 5 natural pigments in adulterated food was developed.In order to determine these constituents by RP-HPLC,cowberry red,red kermel color,black currant red,radish red and grape-skin red were gradient eluted using methanol-acetonitrile-formic acid as mobile phase.Samples were detected simultaneously at the wavelength was 515 nm.Result showed that the relative standard deviation,average recovery and detection limit were 5.7％-7.4％,74.1％-94.7％,5.6-7.5mg/L,respectively.The HPLC method to simultaneously determine five natural pigments was simple,fast,accurate,and reliable with good repeatability.
出处
《中国酿造》
CAS
2014年第7期105-108,共4页
China Brewing
基金
河北省技术监督局项目(2011ZB16)
关键词
掺伪
鉴别
检测
天然色素
adulteration
distinguishing
detection
natural pigment
